Description

3'-Deoxyadenosine 5'-Triphosphate (dATP) is a critical deoxyribonucleotide essential for DNA synthesis and molecular biology research. This high-purity nucleotide analog serves as a vital building block for enzymatic DNA polymerization, ensuring accurate and efficient replication. It is primarily required by biotechnology firms, pharmaceutical R&D laboratories, and academic research institutions for applications in PCR, DNA sequencing, site-directed mutagenesis, and other nucleic acid amplification techniques.

Application

• Polymerase Chain Reaction (PCR): Used as a deoxyribonucleotide triphosphate (dNTP) substrate for DNA amplification by thermostable DNA polymerases.
• DNA Sequencing: Essential component in Sanger sequencing and next-generation sequencing (NGS) workflows for chain elongation.
• cDNA Synthesis: Acts as a raw material in reverse transcription reactions for first-strand cDNA synthesis from RNA templates.
• Site-Directed Mutagenesis: Incorporated into oligonucleotides to introduce specific point mutations during gene editing studies.
• DNA Labeling: Can be used in enzymatic labeling techniques for producing non-radioactive or radioactive DNA probes.
• Enzymatic Assays: Serves as a substrate for assaying the activity of various DNA polymerases, kinases, and other nucleic acid-modifying enzymes.
• Biochemical Research: Fundamental reagent for studying DNA replication, repair mechanisms, and nucleic acid metabolism in vitro.

Basic Information

Product Name	3'-Deoxyadenosine 5'-Triphosphate
CAS No.	73-04-1
Molecular Formula	C10H16N5O12P3
Molecular Weight	491.18 g/mol
Synonyms	2'-Deoxyadenosine-5'-triphosphate; dATP; Deoxyadenosine triphosphate; 2'-Deoxy-5'-ATP; Adenine deoxyribonucleoside triphosphate; 9-(2-Deoxy-β-D-erythro-pentofuranosyl)adenine 5'-(tetrahydrogen triphosphate); (2'R)-2'-Deoxyadenosine 5'-(tetrahydrogen triphosphate)
EINECS	200-778-7

Quality Control

Our 3'-Deoxyadenosine 5'-Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive molecular biology applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We ensure compliance with relevant industry standards for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and aliquoting to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90%
pH (1% Solution)	6.0 - 7.5
Water Content (KF)	≤ 5.0%
Endotoxin	< 10 EU/mg
Solubility	Soluble in water, slightly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.