Description

2’-o-Acetyl-N4-Benzoyl-5’-o-(4,4’-Dimethoxytrityl) Arabinocytidine 3’-o-Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is critically important for the automated production of antisense oligonucleotides, siRNA, and other therapeutic nucleic acids, where precise control over sequence and purity is paramount. It is a key building block for pharmaceutical R&D, biotechnology companies, and diagnostic developers working on advanced genetic medicines and molecular tools.

Application

• Solid-Phase Oligonucleotide Synthesis: Primary monomer for the automated synthesis of arabinose-containing oligonucleotide sequences.
• Therapeutic Nucleic Acid Development: Critical for manufacturing antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) drug candidates.
• Research & Diagnostic Probes: Used to synthesize labeled or modified probes for genetic testing, hybridization assays, and basic molecular biology research.
• Synthesis of Aptamers: Enables the production of structured, nuclease-resistant aptamers for therapeutic and diagnostic applications.
• Process Development & Scale-Up: Serves as a critical raw material for process development and GMP-scale manufacturing of oligonucleotide APIs.

Basic Information

Product Name	2’-o-Acetyl-N4-Benzoyl-5’-o-(4,4’-Dimethoxytrityl) Arabinocytidine 3’-o-Phosphoramidite
CAS No.	675573-97-4
Molecular Formula	C49H49N4O10P
Molecular Weight	893.91 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-acetyl-N4-benzoyl-cytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Ara-C Phosphoramidite (protected); Arabinocytidine Phosphoramidite; 2'-O-Acetyl-5'-O-DMT-arabinocytidine 3'-CE Phosphoramidite; DMT-Ara-C(Bz)-CE Phosphoramidite; Cytidine, 2'-(acetyloxy)-N-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-O-[2-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]ethyl]-; 2'-O-Acetyl-5'-O-(4,4'-dimethoxytrityl)-N4-benzoyl-cytidine-3'-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite
EINECS	Contact for details

Quality Control

Our 2’-o-Acetyl-N4-Benzoyl-5’-o-(4,4’-Dimethoxytrityl) Arabinocytidine 3’-o-Phosphoramidite is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We support development through commercial scale with a commitment to cGMP principles where applicable, ensuring reliable supply for critical pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.