Description

N4-Benzoyl-3',5'-o-(1,1,3,3-Tetraisopropyl-1,3-Disiloxanediyl)Cytidine is a key protected nucleoside derivative, specifically engineered for advanced oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and protecting the cytidine base during automated solid-phase synthesis. It is an essential building block for research and production in antisense oligonucleotides, siRNA, and diagnostic probe development. Our supply guarantees the high purity and batch-to-batch consistency required for sensitive pharmaceutical applications.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Serves as a protected phosphoramidite precursor for the controlled incorporation of cytidine into DNA/RNA strands.
• Antisense & siRNA Therapeutics: A fundamental raw material in the synthesis of therapeutic oligonucleotides targeting genetic diseases and viral infections.
• Diagnostic Probe Manufacturing: Used to produce labeled probes for PCR, FISH, and microarray technologies.
• Research & Development: Enables academic and industrial research into nucleic acid chemistry, aptamer development, and gene function studies.
• Scale-Up cGMP Production: Suitable for use in current Good Manufacturing Practice (cGMP) environments for the production of active pharmaceutical ingredients (APIs) in oligonucleotide drugs.
• Custom Oligo Synthesis Services: A critical reagent for CROs and CDMOs offering custom oligonucleotide synthesis.

Basic Information

Item	Details
Product Name	N4-Benzoyl-3',5'-o-(1,1,3,3-Tetraisopropyl-1,3-Disiloxanediyl)Cytidine
CAS No.	69304-43-4
Molecular Formula	C30H47N3O6Si2
Molecular Weight	609.89 g/mol
Synonyms	N4-Benzoyl-3',5'-O-(1,1,3,3-tetraisopropyl-1,3-disiloxanediyl)cytidine; 1-[3,5-Bis-O-[(1,1,3,3-tetraisopropyl-1,3-disiloxanediyl)]-β-D-ribofuranosyl]-4-benzamidopyrimidin-2(1H)-one; N4-Benzoyl-3',5'-O-(tetraisopropyldisiloxane-1,3-diyl)cytidine; TIPDS-Cyt(Bz); N-Bz-3',5'-O-TIPDS-Cytidine; 3',5'-O-(1,1,3,3-Tetraisopropyl-1,3-disiloxanediyl)-N4-benzoylcytidine; N4-Benzoyl-2'-deoxy-3',5'-O-(1,1,3,3-tetraisopropyldisiloxane-1,3-diyl)cytidine (common misnomer, often used interchangeably); Cytidine, N4-benzoyl-3',5'-O-(1,1,3,3-tetraisopropyl-1,3-disiloxanediyl)-
EINECS	Contact for details

Quality Control

Our production of N4-Benzoyl-3',5'-o-(1,1,3,3-Tetraisopropyl-1,3-Disiloxanediyl)Cytidine adheres to stringent quality protocols suitable for pharmaceutical intermediates. Each batch is analyzed using advanced chromatographic and spectroscopic methods to ensure identity, purity, and stability. Certificates of Analysis (COA) detailing purity (typically ≥98% by HPLC), moisture content, and related substances are provided with every shipment. We support compliance with ICH guidelines for stability and impurity profiling.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled under a dry inert atmosphere (e.g., nitrogen or argon) when opening to prevent degradation. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Related Substances (HPLC)	Total impurities ≤ 2.0%; Any single unknown impurity ≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.