Description

3'-o-Methyluridine-5'-Triphosphate Lithium Salt is a chemically modified nucleotide triphosphate analog, specifically a methylated derivative of uridine triphosphate. This modification is critical for applications requiring nucleotide analogs resistant to enzymatic degradation or for probing RNA structure and function. It is an essential biochemical reagent for researchers and developers in the fields of molecular biology, enzymology, and nucleic acid therapeutics. The compound is supplied as a lithium salt to enhance its stability and solubility in aqueous buffers.

Application

• Substrate for RNA Polymerases: Used in in vitro transcription and RNA synthesis studies to investigate enzyme kinetics, fidelity, and inhibition.
• RNA Structure Probing: Acts as a tool nucleotide to study RNA folding, tertiary interactions, and ribozyme mechanisms.
• Synthesis of Modified RNA Oligonucleotides: Incorporated into synthetic RNA strands to confer nuclease resistance or alter physicochemical properties for therapeutic and diagnostic applications.
• Biochemical Assay Development: Serves as a key component in high-throughput screening assays for discovering antiviral agents or polymerase inhibitors.
• Enzymology Research: Utilized to study the substrate specificity and catalytic mechanisms of nucleotide-processing enzymes like kinases and phosphatases.
• Next-Generation Sequencing (NGS): Potential use as a terminator or modified nucleotide in novel sequencing chemistries.

Basic Information

Product Name	3'-o-Methyluridine-5'-Triphosphate Lithium Salt
CAS No.	69113-65-1
Molecular Formula	C11H16Li3N2O15P3 (or similar, based on salt form)
Molecular Weight	Contact for details
Synonyms	3'-O-Methyl-UTP Lithium Salt; 3'-Methyluridine-5'-triphosphate Li salt; m3'UTP Li Salt; 1-((2R,3R,4R,5R)-3,4-Dihydroxy-5-(6-hydroxy-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-1-yl)-3-methyltetrahydrofuran-2-yl) 1-(trihydrogen triphosphate) Lithium Salt; Uridine, 3'-O-methyl-, 5'-(trihydrogen triphosphate), lithium salt; 3'-OMe-UTP Li+
EINECS	Contact for details

Quality Control

Every batch of 3'-o-Methyluridine-5'-Triphosphate Lithium Salt is manufactured and tested under a strict quality management system. We employ advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and consistency. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.