Description

5-Ethynyl-2'-Deoxycytidine is a chemically modified nucleoside analog, a critical building block in advanced biochemical research and development. Its unique ethynyl functional group makes it a versatile reagent for click chemistry, enabling precise labeling and conjugation of biomolecules. This high-purity compound is essential for researchers and manufacturers in the fields of nucleic acid chemistry, medicinal chemistry, and diagnostic probe development, where reliable quality and structural integrity are paramount.

Application

• Click Chemistry Reagent: Used as a key component in copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions for bioconjugation and labeling.
• Nucleic Acid Research: Serves as a modified nucleoside for the synthesis of oligonucleotide probes and primers with enhanced functionality.
• Medicinal Chemistry & Drug Discovery: Employed in the development of nucleoside-based antiviral and anticancer prodrugs.
• Diagnostic Development: Integral for creating labeled nucleotides used in next-generation sequencing (NGS) and fluorescence in situ hybridization (FISH) assays.
• Biomolecular Labeling: Facilitates the site-specific tagging of DNA, RNA, and other biomolecules for tracking and detection studies.
• Chemical Biology Tool: Used to study nucleic acid metabolism, replication, and repair mechanisms within cells.

Basic Information

Product Name	5-Ethynyl-2'-Deoxycytidine
CAS No.	69075-47-4
Molecular Formula	C11H13N3O4
Molecular Weight	251.24 g/mol
Synonyms	5-Ethynyl-2'-deoxycytidine; 5-Ethynyl-dC; EdC; 4-Amino-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidin-2(1H)-one; 5-Ethynylcytidine deoxyriboside; 2'-Deoxy-5-ethynylcytidine; 1-(2-Deoxy-β-D-erythro-pentofuranosyl)-5-ethynylcytosine
EINECS	Contact for details

Quality Control

Our 5-Ethynyl-2'-Deoxycytidine is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high chemical purity, typically meeting standards suitable for research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We are committed to supporting our clients' regulatory and quality assurance needs, with specifications available for review.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants or an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 10 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.