Description

5-Ethynyl Uridine is a modified nucleoside analog featuring an ethynyl group at the 5-position of the uracil base, offering a versatile handle for bioorthogonal chemistry. This compound is a critical building block for the synthesis of labeled nucleotides and oligonucleotides, enabling advanced research in molecular biology and diagnostics. It is essential for scientists and manufacturers in the fields of pharmaceutical research, diagnostic probe development, and chemical biology, where precise molecular tagging and detection are required.

Application

• Click Chemistry Reagent: Acts as a key substrate for copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions to label nucleic acids.
• Probe Synthesis: Used in the development of fluorescent or affinity-labeled nucleotide probes for detecting RNA and DNA.
• Antiviral Research: Serves as a precursor or intermediate in the synthesis of nucleoside analogs with potential antiviral activity.
• Chemical Biology Tools: Incorporated into oligonucleotides to study RNA structure, function, and protein interactions.
• Diagnostic Assays: Utilized in the manufacturing of components for molecular diagnostic kits and sequencing technologies.
• Bioconjugation: Enables the site-specific attachment of biomolecules, polymers, or surfaces via its reactive alkyne group.

Basic Information

Product Name	5-Ethynyl Uridine
CAS No.	69075-42-9
Molecular Formula	C11H12N2O6
Molecular Weight	268.22 g/mol
Synonyms	5-Ethynyluridine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-5-ethynylpyrimidine-2,4-dione; 5-Ethynyl-1-β-D-ribofuranosyluracil; 5-Ethynyl-1-β-D-ribosyluracil; Uridine, 5-ethynyl-
EINECS	Contact for details

Quality Control

Our 5-Ethynyl Uridine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and NMR for structural confirmation, to ensure it meets the high standards required for research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccant and inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.