Description

2'-Deoxytubercidin-5'-Triphosphate is a chemically modified nucleoside triphosphate analog, a critical building block for advanced biochemical research and development. This compound is valued for its ability to act as a chain terminator or substrate analog in enzymatic studies, particularly those involving DNA polymerases and reverse transcriptases. It is primarily utilized by research institutions and biotechnology companies focused on virology, oncology, and nucleic acid chemistry for applications in sequencing, probe synthesis, and mechanistic enzymology.

Application

• Nucleic Acid Sequencing & Analysis: Used as a substrate analog in Sanger sequencing and next-generation sequencing platforms to study polymerase fidelity and mechanism.
• Enzymology Research: Serves as a chain-terminating nucleotide or inhibitor in kinetic studies of DNA-dependent and RNA-dependent polymerases.
• Antiviral & Anticancer Research: Investigated for its potential to inhibit viral replication (e.g., HIV reverse transcriptase) and cellular processes in oncology target validation.
• Probe & Primer Synthesis: Employed in the enzymatic synthesis of labeled or modified nucleic acid probes for hybridization and diagnostic assays.
• Biochemical Assay Development: Acts as a critical reagent in high-throughput screening assays to identify novel polymerase inhibitors or modulators.
• Metabolic Pathway Studies: Used to trace and understand the incorporation and metabolism of nucleotide analogs within cellular systems.

Basic Information

Product Name	2'-Deoxytubercidin-5'-Triphosphate
CAS No.	67460-15-5
Molecular Formula	C15H23N5O13P3
Molecular Weight	598.28 g/mol
Synonyms	dTTP-7-deaza analog; 7-Deaza-2'-deoxyguanosine-5'-triphosphate; Tubercidin 5'-triphosphate 2'-deoxy; 2'-Deoxy-7-deazaguanosine triphosphate; 4-Amino-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-7H-pyrrolo[2,3-d]pyrimidine-5-carboxamide 5'-(trihydrogen triphosphate); 2'-Deoxy-7-deazaguanosine-5'-triphosphate; d7GTP
EINECS	Contact for details

Quality Control

Every batch of 2'-Deoxytubercidin-5'-Triphosphate is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure the highest standards of purity and consistency required for sensitive research applications. Our quality assurance protocol includes advanced chromatographic and spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water or aqueous buffer
Biological Activity	Tested in standard polymerase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.