Description

5'-O-Dimethoxytrityl-N-Benzoyl-Deoxycytidine is a key phosphoramidite monomer used in the solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA and RNA strands. It is an essential raw material for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries developing therapeutics, probes, and primers.

Application

• Oligonucleotide Synthesis: Primary building block (phosphoramidite monomer) for the automated solid-phase synthesis of DNA and RNA sequences.
• Therapeutic Development: Manufacture of antisense oligonucleotides (ASOs), siRNA, and aptamers for targeted drug discovery and development.
• Research Reagents: Production of custom primers, probes, and gene fragments for PCR, sequencing, and molecular biology research.
• Diagnostic Kits: Synthesis of labeled probes and capture sequences used in diagnostic assays and genomic testing.
• Scale-Up GMP Production: Serves as a starting material for the large-scale, Good Manufacturing Practice (GMP) compliant production of oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	5'-O-Dimethoxytrityl-N-Benzoyl-Deoxycytidine
CAS No.	67219-55-0
Molecular Formula	C37H35N3O7
Molecular Weight	633.70 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine; DMT-dC(Bz) Phosphoramidite precursor; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine; 5'-O-DMT-N-Bz-deoxycytidine; dC(Bz) DMT; N-Bz-5'-O-DMT-2'-deoxycytidine
EINECS	Contact for details

Quality Control

Our 5'-O-Dimethoxytrityl-N-Benzoyl-Deoxycytidine is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the stringent requirements for oligonucleotide synthesis. Certificates of Analysis (COA) with lot-specific data are provided to guarantee reproducible performance and supply chain traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. This material is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere (argon or nitrogen) after opening to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.