Description

2',3'-Dideoxycytidine 5'-Triphosphate (CAS NO 66004-77-1) is a modified nucleoside triphosphate analog critical for advanced molecular biology and biochemistry research. Its primary value lies in its ability to act as a chain terminator in enzymatic DNA synthesis, making it an indispensable tool for sequencing and related applications. This high-purity biochemical is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable, high-quality reagents for nucleic acid analysis and development.

Application

• DNA Sequencing: Used as a chain-terminating substrate in Sanger sequencing and next-generation sequencing (NGS) platforms.
• Enzymatic Synthesis: Serves as a modified building block for the controlled synthesis of oligonucleotides with specific terminal modifications.
• Biochemical Research: A key reagent in studies of DNA polymerase kinetics, fidelity, and mechanism of action.
• Antiviral Research: Investigated as a potential nucleotide analog for probing viral reverse transcriptase inhibition mechanisms.
• Diagnostic Assay Development: Utilized in the design and production of specialized probes and primers for molecular diagnostics.
• Quality Control (QC) Standards: Acts as a reference standard in the QC of sequencing kits and other molecular biology reagents.

Basic Information

Product Name	2',3'-Dideoxycytidine 5'-Triphosphate
CAS No.	66004-77-1
Molecular Formula	C9H15N3O12P3
Molecular Weight	474.14 g/mol
Synonyms	ddCTP; 2',3'-Dideoxycytidine triphosphate; 2',3'-ddCTP; Cytidine 5'-(tetrahydrogen triphosphate), 2',3'-dideoxy-; 2',3'-Dideoxycytidine 5'-(tetrahydrogen triphosphate); ddC-TP; Dideoxycytidine triphosphate; 2',3'-Dideoxy-CTP
EINECS	Contact for details

Quality Control

Our 2',3'-Dideoxycytidine 5'-Triphosphate is manufactured under stringent conditions to ensure the highest standards of purity and consistency required for sensitive research and development. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to guarantee traceability and compliance with your quality protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, slightly soluble in methanol
Biological Activity	Suitable for use as a chain terminator in DNA polymerase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.