Description

(25R)-3β-[[2-o-(α-L-Rhamnopyranosyl)-4-o-(α-L-Arabinofuranosyl)-β-D-Glucopyranosyl]Oxy]Spirosta-5-Ene-17-Ol CAS NO 65607-36-5 is a high-purity steroidal saponin glycoside, a complex natural product derived from botanical sources. This compound is valued for its role as a critical reference standard and active pharmaceutical ingredient (API) intermediate in advanced research and pharmaceutical development. It is primarily required by pharmaceutical R&D laboratories, nutraceutical manufacturers, and academic research institutions focused on metabolic and inflammatory pathways.

Application

• Pharmaceutical Reference Standard: Used for the qualitative and quantitative analysis of steroidal saponins in drug development and quality control.
• Active Pharmaceutical Ingredient (API) Intermediate: Serves as a key building block in the synthesis of novel therapeutic agents.
• Biochemical Research: Employed in in-vitro and in-vivo studies to investigate biological activities, including anti-inflammatory and metabolic effects.
• Nutraceutical Formulation: Incorporated into high-end dietary supplements targeting specific health benefits, requiring verified purity.
• Analytical Method Development: Essential for calibrating instruments and validating HPLC, LC-MS, and other chromatographic methods.
• Natural Product Isolation & Standardization: Acts as a benchmark for standardizing plant extracts and ensuring batch-to-batch consistency.

Basic Information

Product Name	(25R)-3β-[[2-o-(α-L-Rhamnopyranosyl)-4-o-(α-L-Arabinofuranosyl)-β-D-Glucopyranosyl]Oxy]Spirosta-5-Ene-17-Ol
CAS No.	65607-36-5
Molecular Formula	C₄₄H₇₀O₁₇
Molecular Weight	871.0 g/mol
Synonyms	Timosaponin B-II; (25R)-Spirost-5-en-3β-ol 3-O-{β-D-glucopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→4)]-β-D-glucopyranoside}; Anemarrhena Saponin B-II; Steroidal Saponin B-II; 3-O-{β-D-Glucopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→4)]-β-D-glucopyranosyl}-(25R)-spirost-5-en-3β-ol; SB-II; Contact for additional aliases.
EINECS	Contact for details

Quality Control

Our production of (25R)-3β-[[2-o-(α-L-Rhamnopyranosyl)-4-o-(α-L-Arabinofuranosyl)-β-D-Glucopyranosyl]Oxy]Spirosta-5-Ene-17-Ol adheres to stringent quality protocols suitable for research and pharmaceutical applications. Each batch is characterized and tested using advanced analytical techniques including HPLC, LC-MS, and NMR to ensure identity, purity, and consistency. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your regulatory compliance and research integrity.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after opening to prevent absorption of atmospheric moisture. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Specific Rotation	Contact for details
Single Impurity (HPLC)	≤ 1.0%
Total Impurities (HPLC)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.