Description

[(25R)-Spirostane-5-Ene-3β-Yl]3-o-β-D-Xylopyranosyl-β-D-Glucopyranoside CAS NO 65604-99-1 is a high-purity steroidal saponin glycoside, a compound of significant interest in advanced pharmaceutical and biochemical research. Its value lies in its specific stereochemistry and glycosidic linkage, which are critical for its biological activity and utility as a reference standard or synthetic intermediate. This product is essential for researchers and manufacturers in the pharmaceutical, nutraceutical, and life science sectors who require well-characterized, high-quality bioactive compounds for drug discovery, analytical method development, and quality control.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the identification, assay, and impurity profiling of steroidal saponin-based drugs and herbal extracts in quality control laboratories.
• Biochemical Research: Serves as a key intermediate or substrate in enzymatic studies, metabolic pathway investigations, and structure-activity relationship (SAR) studies for novel therapeutics.
• Natural Product Isolation & Standardization: Employed as a marker compound for the standardization and authentication of botanical extracts containing similar spirostane-type saponins.
• Academic & Institutional Research: Utilized in universities and research institutes for pharmacological screening, including anti-inflammatory, anti-cancer, or neuroprotective activity assays.
• Analytical Method Development: Acts as a critical component for developing and validating analytical methods such as HPLC, LC-MS, and NMR for complex natural product matrices.
• Nutraceutical Development: Investigated for potential incorporation into high-end dietary supplements where standardized bioactive saponin content is required.

Basic Information

Item	Detail
Product Name	[(25R)-Spirostane-5-Ene-3β-Yl]3-o-β-D-Xylopyranosyl-β-D-Glucopyranoside
CAS No.	65604-99-1
Molecular Formula	C38H60O12
Molecular Weight	708.88 g/mol
Synonyms	(25R)-Spirost-5-en-3β-yl 3-O-β-D-xylopyranosyl-β-D-glucopyranoside; Dioscin (a primary steroidal saponin often associated with this structure); 3-O-[β-D-Xylopyranosyl-(1→3)-β-D-glucopyranosyl]-25R-spirost-5-en-3β-ol; Triterpenoid saponin glycoside; Steroidal glycoside; Dioscorea saponin derivative; Contact for additional trade names and abbreviations.
EINECS	Contact for details

Quality Control

Every batch of [(25R)-Spirostane-5-Ene-3β-Yl]3-o-β-D-Xylopyranosyl-β-D-Glucopyranoside is manufactured and tested under a strict quality management system. Our products undergo rigorous quality testing to ensure compliance with industry standards for research and pharmaceutical applications. Comprehensive analytical techniques, including HPLC, NMR, and MS, are employed to confirm identity, purity, and consistency. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.