Description

5-Ethynyl Cytidine is a modified nucleoside analog featuring an ethynyl group at the 5-position of the cytosine base. This structural modification is crucial for its role as a key intermediate and building block in the synthesis of novel oligonucleotides and therapeutic agents. It is primarily utilized by research institutions and pharmaceutical companies engaged in nucleic acid chemistry, antiviral drug development, and the creation of diagnostic probes. The compound's alkyne functionality enables efficient click chemistry reactions, making it a versatile tool for bioconjugation and labeling applications.

Application

• Pharmaceutical Intermediate: Critical building block for the synthesis of novel antiviral and anticancer nucleoside analogs.
• Oligonucleotide Synthesis: Used in the preparation of modified RNA and DNA strands for research and therapeutic purposes (e.g., antisense oligonucleotides, siRNA).
• Bioconjugation & Labeling: The ethynyl group allows for site-specific labeling of biomolecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry.
• Proteomics & Genomics Research: Serves as a metabolic label for tracking nucleic acid synthesis and turnover in cellular systems.
• Diagnostic Probe Development: Incorporated into probes for detecting specific DNA/RNA sequences or studying nucleic acid-protein interactions.
• Chemical Biology Tool: Enables the study of cellular processes involving nucleic acids through bioorthogonal chemistry strategies.

Basic Information

Product Name	5-Ethynyl Cytidine
CAS No.	65223-78-1
Molecular Formula	C11H13N3O5
Molecular Weight	267.24 g/mol
Synonyms	5-Ethynylcytidine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-5-ethynylpyrimidine-2,4-dione; 5-Ethynyl-1-β-D-ribofuranosylcytosine; Cytidine, 5-ethynyl-; 5-Ethynyl-1-β-D-ribofuranosyl-2(1H)-pyrimidinone
EINECS	Contact for details

Quality Control

Our 5-Ethynyl Cytidine is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and NMR for structural confirmation. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment. We adhere to cGMP guidelines where applicable to support our clients in research and development phases.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Keep the container tightly sealed in a desiccated environment after opening.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.