Description

4-Azidophenacyl Iodoacetate is a specialized bifunctional crosslinking reagent featuring both a photoreactive azido group and an iodoacetyl group. This compound is critical for researchers developing photoaffinity labeling probes and site-specific bioconjugation strategies, enabling the covalent attachment of molecules to biological targets under controlled UV light activation. It is primarily needed by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic kit manufacturers working on protein-protein interaction studies, target identification, and the development of novel therapeutic conjugates.

Application

• Photoaffinity Labeling: A key reagent for covalently tagging proteins, nucleic acids, or other biomolecules to study binding sites and molecular interactions upon UV irradiation.
• Bioconjugation & Probe Synthesis: Used to create custom chemical probes by conjugating reporter molecules (e.g., fluorophores, biotin) or drugs to specific target sites via the iodoacetyl group.
• Chemical Biology Research: Facilitates the study of enzyme mechanisms, receptor-ligand interactions, and cellular pathways through irreversible crosslinking.
• Proteomics & Target Identification: Employed in activity-based protein profiling (ABPP) to identify and characterize active sites within complex proteomes.
• Material Surface Functionalization: Can be used to modify surfaces with bioactive molecules for biosensor development or tissue engineering scaffolds.
• Antibody-Drug Conjugate (ADC) Research: Serves as a tool compound in early-stage research for exploring novel linker chemistries in targeted cancer therapies.

Basic Information

Product Name	4-Azidophenacyl Iodoacetate
CAS No.	65116-98-5
Molecular Formula	C10H8IN3O3
Molecular Weight	345.09 g/mol
Synonyms	N-(4-Azidophenacyl)iodoacetamide; 2-Iodo-N-(2-oxo-2-(4-azidophenyl)ethyl)acetamide; 4-Azidophenacyl iodoacetamide; Iodoacetic Acid 4-Azidophenacyl Ester; p-Azidophenacyl Iodoacetate; α-Iodoacetamido-4-azidoacetophenone; Photoactivatable Iodoacetyl Crosslinker
EINECS	Contact for details

Quality Control

Our 4-Azidophenacyl Iodoacetate is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and impurity profiles. We adhere to ISO 9001 quality management standards, and our production environment is designed to prevent cross-contamination. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This compound is strictly light-sensitive and must be handled under subdued light or amber glass to prevent premature photolysis. Allow the container to reach room temperature in a dark environment before opening to minimize condensation.

Specification

Item	Specification
Appearance	Off-white to pale yellow crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.