Description

25-Hydroxyvitamin D is a crucial metabolite of Vitamin D, serving as the primary circulating form and the most reliable clinical indicator of an individual's Vitamin D status. Its accurate quantification is essential for diagnosing and managing deficiencies, making it a high-value reference standard and raw material. This compound is primarily required by pharmaceutical manufacturers, clinical diagnostic kit producers, and research institutions focused on endocrinology, bone health, and nutritional science.

Application

• Pharmaceutical Reference Standard: Used as a primary standard in the quality control and assay development of Vitamin D-based medications and supplements.
• Clinical Diagnostics: Critical component in the calibration and production of immunoassay kits (e.g., ELISA, CLIA) for measuring serum 25-Hydroxyvitamin D levels.
• Biochemical Research: Employed in studies investigating Vitamin D metabolism, receptor binding, and its role in calcium homeostasis, immune function, and cell regulation.
• Nutraceutical Development: Serves as a high-purity ingredient in the formulation of advanced dietary supplements targeting specific health outcomes.
• Veterinary Medicine: Used in diagnostic and therapeutic applications for animal health, particularly in livestock and companion animals.

Basic Information

Item	Detail
Product Name	25-Hydroxyvitamin D
CAS No.	64719-49-9
Molecular Formula	C27H44O2
Molecular Weight	400.64 g/mol
Synonyms	Calcifediol; 25-Hydroxycholecalciferol; 25-OH Vitamin D3; 25(OH)D; 25-Hydroxy Vitamin D3; Pre-vitamin D; Ro 21-5535
EINECS	Contact for details

Quality Control

Our 25-Hydroxyvitamin D is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. We provide Certificates of Analysis (COA) with detailed chromatograms and full traceability. The product is suitable for use under cGMP guidelines for pharmaceutical and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. The container should be kept in a cool, dry, and well-ventilated area. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.