Description

Guanylyl-5'-Imidodiphosphate, Tetralithium Salt is a high-purity, non-hydrolyzable analog of GTP (guanosine triphosphate) that acts as a potent inhibitor of G-proteins and other GTP-binding proteins. This compound is critical for biochemical research, enabling the study of signal transduction pathways by locking proteins in their active or inactive states. It is an essential reagent for molecular biologists, biochemists, and pharmaceutical researchers investigating cellular regulation, enzyme kinetics, and drug discovery targets.

Application

• Biochemical Research: Used as a non-hydrolyzable GTP analog to study the mechanism of GTP-binding proteins (G-proteins, Ras, EF-Tu) and signal transduction cascades.
• Enzyme Inhibition Studies: Serves as a potent inhibitor to characterize the kinetics and regulation of GTPases, ATPases, and nucleotide-dependent enzymes.
• Structural Biology: Employed in X-ray crystallography and cryo-EM to stabilize specific conformational states of proteins for structural analysis.
• Drug Discovery & Screening: Utilized in high-throughput screening (HTS) assays to identify novel modulators of GTPase activity for therapeutic development.
• Cell Signaling Pathway Analysis: A key tool for dissecting the role of G-proteins in cellular processes like proliferation, differentiation, and vesicular transport.
• Diagnostic Kit Development: Incorporated into in vitro diagnostic (IVD) kits for research use to measure specific enzymatic activities.

Basic Information

Product Name	Guanylyl-5'-Imidodiphosphate, Tetralithium Salt
CAS No.	64564-03-0
Molecular Formula	C10H12Li4N6O13P3
Molecular Weight	568.02 g/mol
Synonyms	Guanosine 5'-(β,γ-imido)triphosphate, Tetralithium Salt; Gpp(NH)p, Tetralithium Salt; 5'-Guanylyl imidodiphosphate, Tetralithium Salt; Guanosine-5'-[β,γ-imido]triphosphate, Tetralithium Salt; GTPγS Non-hydrolyzable Analog; GMP-PNP, Tetralithium Salt; Guanylyl Imidodiphosphate Li Salt
EINECS	Contact for details

Quality Control

Our Guanylyl-5'-Imidodiphosphate, Tetralithium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high biological activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard biochemical assays. We support research and development with materials suitable for critical applications, ensuring reliability for your most demanding experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Biological Activity	Conforms to reference standard in inhibition assay
Endotoxin	< 10 EU/mg (if specified for biological use)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.