Description

5-Ethyl-2'-Deoxyuridine Triphosphate is a modified nucleoside triphosphate analog, a critical building block for advanced molecular biology and diagnostic applications. Its value lies in its ability to be incorporated into nucleic acid chains, enabling precise research and development in areas requiring sequence-specific probes or modified DNA. This compound is essential for researchers and manufacturers in the fields of molecular diagnostics, antiviral research, and next-generation sequencing. Its specific modification offers unique properties for probing enzyme mechanisms and developing novel therapeutic and diagnostic agents.

Application

• Molecular Diagnostics: Used as a substrate in enzymatic assays and for the synthesis of labeled probes for detecting specific DNA sequences.
• Antiviral Research: Serves as a potential chain terminator or inhibitor in studies targeting viral polymerases, such as herpesviruses.
• Next-Generation Sequencing (NGS): Employed in specialized sequencing protocols that utilize modified nucleotides to study DNA replication or for novel sequencing chemistries.
• Biochemical Research: Acts as a tool compound to study the kinetics and specificity of DNA polymerases and other nucleic acid-processing enzymes.
• Probe Synthesis: Integral for creating non-radioactive or fluorescently tagged nucleic acid probes used in FISH (Fluorescence In Situ Hybridization) and microarray technologies.
• Drug Discovery: Used in high-throughput screening assays to identify new polymerase inhibitors for therapeutic development.

Basic Information

Product Name	5-Ethyl-2'-Deoxyuridine Triphosphate
CAS No.	64406-41-3
Molecular Formula	C12H18N3O13P3
Molecular Weight	505.21 g/mol
Synonyms	5-Ethyl-dUTP; EdUTP; 5-Ethyl-2'-deoxyuridine 5'-triphosphate; 5-Ethyl-2'-deoxyuridine-5'-triphosphate; 2'-Deoxy-5-ethyluridine triphosphate; 5-Ethyl-deoxyuridine triphosphate; d5-EthylUTP
EINECS	Contact for details

Quality Control

Our 5-Ethyl-2'-Deoxyuridine Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Quality is verified through comprehensive analytical methods including HPLC, NMR, and enzymatic activity assays. A detailed Certificate of Analysis (COA) is provided with each batch, confirming identity, purity, and absence of contaminating nucleases.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Enzymatic Incorporation Assay	Suitable for use
Nuclease Contamination	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.