Description

5-n-Hexyl-2'-Deoxyuridine Triphosphate is a modified nucleotide triphosphate analog designed for advanced biochemical and molecular biology research. This compound is critical for applications requiring the stable incorporation of a hexyl-modified base, offering enhanced properties for studying nucleic acid interactions and enzyme mechanisms. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical companies engaged in next-generation sequencing, probe development, and enzymatic studies.

Application

• Next-Generation Sequencing (NGS): Used as a modified dUTP analog for specialized sequencing protocols and mutagenesis studies.
• Polymerase Chain Reaction (PCR): Employed in the synthesis of hexyl-labeled DNA probes for sensitive detection and hybridization assays.
• Enzymology Studies: Serves as a substrate analog to investigate the kinetics and specificity of DNA polymerases and other nucleic acid-processing enzymes.
• Diagnostic Assay Development: Integral for creating labeled nucleotides used in fluorescence in situ hybridization (FISH) and other molecular diagnostic platforms.
• Antiviral Research: Investigated for its potential as a chain terminator or inhibitor in studies targeting viral replication mechanisms.
• Bioconjugation and Labeling: The hexyl side chain provides a handle for further chemical modification and attachment of reporter molecules.

Basic Information

Product Name	5-n-Hexyl-2'-Deoxyuridine Triphosphate
CAS No.	64374-81-8
Molecular Formula	C16H26N3O14P3
Molecular Weight	577.33 g/mol
Synonyms	5-Hexyl-dUTP; 5-(n-Hexyl)-2'-deoxyuridine-5'-triphosphate; dUTP-5-Hexyl; 5-n-Hexyldeoxyuridine triphosphate; 2'-Deoxy-5-(hexyl)uridine 5'-triphosphate; Hexyl-modified dUTP; 5-C6-dUTP; CAS 64374-81-8
EINECS	Contact for details

Quality Control

Our 5-n-Hexyl-2'-Deoxyuridine Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and mass spectrometry for identity verification. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Biological Activity	Suitable for enzymatic incorporation (tested)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.