Description

5-Isopropyl-2'-Deoxyuridine Triphosphate is a chemically modified nucleotide triphosphate analog, specifically engineered for advanced molecular biology and biochemical research applications. Its core value lies in its ability to act as a substrate for DNA polymerases, enabling the site-specific incorporation of modified bases into nucleic acid chains, which is crucial for probing enzyme mechanisms and developing diagnostic assays. This high-purity biochemical is essential for researchers and developers in the fields of molecular diagnostics, enzymology, and next-generation sequencing (NGS) probe development, where precise control over nucleic acid structure and function is required.

Application

• Enzymology Studies: Used as a substrate to investigate the kinetics, fidelity, and mechanism of DNA polymerases and reverse transcriptases.
• Molecular Diagnostics: Incorporation into oligonucleotide probes for techniques like PCR, qPCR, and microarray analysis to enhance specificity or introduce labeling sites.
• Next-Generation Sequencing (NGS): Serves as a modified nucleotide for specialized sequencing-by-synthesis applications or probe construction.
• Antiviral Research: Studied for its potential as a chain terminator or inhibitor in the development of nucleoside analog therapeutics.
• Bioconjugation & Labeling: The isopropyl modification provides a handle for further chemical derivatization, enabling the attachment of fluorophores, biotin, or other reporter molecules to DNA.
• Structural Biology: Used to create modified DNA or RNA substrates for X-ray crystallography or NMR studies to understand nucleic acid-protein interactions.

Basic Information

Product Name	5-Isopropyl-2'-Deoxyuridine Triphosphate
CAS No.	64374-79-4
Molecular Formula	C13H20N2O14P3
Molecular Weight	530.22 g/mol
Synonyms	5-Isopropyl-dUTP; 5-(1-Methylethyl)-2'-deoxyuridine 5'-(tetrahydrogen triphosphate); 5-iPr-dUTP; 2'-Deoxy-5-isopropyluridine triphosphate; dITP analog; 5-Prop-2-yl-2'-deoxyuridine triphosphate; Modified nucleotide triphosphate; Isopropyl-dU triphosphate
EINECS	Contact for details

Quality Control

Our 5-Isopropyl-2'-Deoxyuridine Triphosphate is manufactured under stringent quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and mass spectrometry for identity verification. Certificates of Analysis (COA) detailing all specifications are provided to guarantee compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 3.0%
Triphosphate Content (P31 NMR)	≥ 85%
Biological Activity	Suitable for polymerase incorporation assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.