Description

N6-Benzoyl-5'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine is a critical, high-purity phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound matters because it serves as a protected building block for the adenine nucleotide, ensuring high coupling efficiency and fidelity during DNA/RNA sequence assembly. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable, scalable synthesis of primers, probes, and therapeutic oligonucleotides.

Application

• Oligonucleotide Synthesis: Primary building block (phosphoramidite) for the incorporation of adenine (dA) in automated DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug development.
• Diagnostic Probe Production: Synthesis of labeled probes and primers for PCR, qPCR, and next-generation sequencing (NGS) applications.
• Research & Development: Used in academic and industrial labs for custom gene synthesis and molecular biology research.
• Synthesis of Modified Oligonucleotides: A key starting material for creating oligonucleotides with site-specific modifications or labels.
• Scale-Up cGMP Production: Suitable for the manufacture of oligonucleotide active pharmaceutical ingredients (APIs) under current Good Manufacturing Practice (cGMP) guidelines.

Basic Information

Product Name	N6-Benzoyl-5'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine
CAS No.	64325-78-6
Molecular Formula	C44H39N5O6
Molecular Weight	733.82 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-deoxyadenosine; DMTr-dA(Bz)-OH; N6-Benzoyl-2'-deoxyadenosine, 5'-O-(bis(4-methoxyphenyl)phenylmethyl) derivative; dA(Bz) DMTr; 2'-Deoxyadenosine, N6-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; N-Bz-5'-O-DMTr-2'-deoxyadenosine; Bz-dA(DMTr)
EINECS	Contact for details

Quality Control

Our N6-Benzoyl-5'-o-(4,4'-Dimethoxytrityl)-2'-Deoxyadenosine is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure identity, purity, and performance. Certificates of Analysis (COA) with detailed chromatographic data are provided. We support production under ISO 9001 and can supply material suitable for cGMP applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) due to its hygroscopic and light-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DMF, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.