Description

2'-Deoxyadenosine 5'-o-(1-Thiotriphosphate) is a chemically modified nucleotide analog where one of the non-bridging oxygen atoms in the triphosphate moiety is replaced by sulfur. This modification is critical for its primary use as a substrate for DNA polymerases in molecular biology techniques, offering resistance to enzymatic degradation. It is an essential reagent for researchers and manufacturers in the fields of biotechnology, molecular diagnostics, and pharmaceutical development who require high-purity, reliable nucleotide components for enzymatic assays and sequencing applications.

Application

• DNA Sequencing & Analysis: Used as a substrate in Sanger sequencing and next-generation sequencing (NGS) platforms where its incorporation terminates chain elongation, enabling base identification.
• Enzymatic Assays & Biochemistry Research: Serves as a crucial reagent for studying the kinetics and mechanisms of DNA polymerases, reverse transcriptases, and other nucleic acid-processing enzymes.
• Molecular Diagnostics: Employed in the development and production of diagnostic kits for genetic testing, pathogen detection, and SNP analysis.
• Antiviral & Anticancer Drug Development: Acts as a potential precursor or active metabolite in the research of nucleoside analog prodrugs targeting viral replication or cellular proliferation.
• Site-Directed Mutagenesis: Utilized in techniques that introduce specific mutations into DNA sequences for functional genomics studies.
• Labeling & Detection Probes: Can be incorporated into nucleic acid probes, with the thiophosphate group serving as a handle for subsequent labeling or detection strategies.

Basic Information

Product Name	2'-Deoxyadenosine 5'-o-(1-Thiotriphosphate)
CAS No.	64145-28-4
Molecular Formula	C10H16N5O11P3S
Molecular Weight	507.26 g/mol
Synonyms	2'-Deoxyadenosine-5'-O-(1-thiotriphosphate); dATPαS; Deoxyadenosine 5'-(α-thio)triphosphate; Adenosine-5'-(3-thiotriphosphate), 2'-deoxy-; 2'-Deoxy-ATP-α-S; dATP-α-S; (Sp)-2'-Deoxyadenosine-5'-O-(1-thiotriphosphate); Thiophosphoryl deoxyadenosine triphosphate
EINECS	Contact for details

Quality Control

Our 2'-Deoxyadenosine 5'-o-(1-Thiotriphosphate) is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is rigorously tested using advanced analytical techniques including HPLC and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation and storage under inert atmosphere to maintain stability and potency. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Diastereomeric Purity (Sp-isomer)	≥ 98%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, TE buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.