Description

Methyl 3-((4-Azidophenyl)Dithio)Propionimidate CAS NO 63958-36-1 is a specialized heterobifunctional crosslinking reagent containing both a photoactivatable aryl azide group and a cleavable disulfide bond. This compound matters for researchers and manufacturers requiring controlled, reversible conjugation in complex biomolecular systems, such as attaching probes or drugs to proteins or nanoparticles. Professionals in bioconjugation, pharmaceutical development, and advanced materials science need this reagent for its unique ability to form stable linkages that can be selectively cleaved under reducing conditions.

Application

• Bioconjugation and Protein Labeling: For site-specific attachment of fluorescent dyes, affinity tags, or drugs to antibodies, enzymes, and other proteins via the imidoester group, with a cleavable linker.
• Affinity Chromatography Probe Synthesis: Used to immobilize ligands onto solid supports through the disulfide bond, allowing for gentle elution of captured biomolecules.
• Surface Functionalization: Modifying gold nanoparticles, chips, or other materials to create reversible capture surfaces for biosensing and diagnostic applications.
• Prodrug and Drug Delivery System Development: Constructing linker systems where the therapeutic payload is released upon exposure to intracellular reducing environments like glutathione.
• Photoaffinity Labeling: The aryl azide moiety allows for UV light-induced, covalent crosslinking to study protein-protein interactions or ligand-receptor binding sites.
• Polymer Chemistry: Serving as a functional monomer or crosslinker in the synthesis of smart, redox-responsive hydrogels and polymers.

Basic Information

Product Name	Methyl 3-((4-Azidophenyl)Dithio)Propionimidate
CAS No.	63958-36-1
Molecular Formula	C10H10N4OS2
Molecular Weight	266.34 g/mol
Synonyms	Methyl 3-[(4-Azidophenyl)dithio]propionimidate; 4-Azidophenyl 3-(Methoxyimino)propyl Disulfide; 3-((4-Azidophenyl)disulfanyl)-N-methoxypropanimidamide; Methyl 3-(4-Azidophenyldisulfanyl)propionimidate; Azido-PDTP; Heterobifunctional Crosslinker (Azido/Imidoester, cleavable); SSA (Succinimidyl Sulfonyl Azide) related reagent; p-Azidophenyl 3-(Methoxyimino)propyl Disulfide
EINECS	Contact for details

Quality Control

Our Methyl 3-((4-Azidophenyl)Dithio)Propionimidate is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR and IR for structural confirmation, and functional group analysis to ensure optimal reactivity. Certificates of Analysis (COA) detailing batch-specific results are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and degradation. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. Keep away from heat, sparks, and open flame.

Specification

Item	Specification
Appearance	Yellow to light brown solid or oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Functional Group Assay	≥ 90% (Imidoester & Azide)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.