Description

3'-o-Methylguanosine Triphosphate is a chemically modified nucleotide triphosphate analog, specifically a methylated derivative of guanosine triphosphate (GTP). This modification is crucial for studying RNA structure, function, and enzymatic processes, particularly those involving RNA polymerases and GTP-binding proteins. It is an essential research-grade biochemical for molecular biology, enzymology, and pharmaceutical development, serving scientists in academia, biotechnology, and drug discovery.

Application

• Enzymology Research: Used as a substrate analog to investigate the specificity and mechanism of RNA polymerases, GTPases, and other nucleotide-dependent enzymes.
• RNA Studies: Employed in the synthesis of modified RNA oligonucleotides to study RNA folding, stability, and protein-RNA interactions.
• Biochemical Assay Development: Serves as a critical component in high-throughput screening assays for drug discovery targeting nucleotide-binding sites.
• Structural Biology: Aids in X-ray crystallography and NMR studies to determine the three-dimensional structures of enzyme-substrate complexes.
• Diagnostic Reagent Manufacturing: Can be utilized in the production of specialized reagents for molecular diagnostics and next-generation sequencing platforms.

Basic Information

Product Name	3'-o-Methylguanosine Triphosphate
CAS No.	61556-45-4
Molecular Formula	C11H18N5O14P3
Molecular Weight	561.21 g/mol
Synonyms	3'-O-Methyl-GTP; 3'-O-Methylguanosine-5'-triphosphate; 3'-Methyl-GTP; Guanosine-5'-triphosphate, 3'-O-methyl-; 3'-OMe-GTP; m3'-GTP; 3'-O-Methyl GTP trisodium salt (common salt form)
EINECS	Contact for details

Quality Control

Our 3'-o-Methylguanosine Triphosphate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and mass spectrometry for identity verification. We provide Certificates of Analysis (COA) with detailed specifications for all critical parameters, ensuring consistency and reliability for your research. The product meets high-purity standards suitable for biochemical and pharmaceutical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, it is recommended to store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0%
pH (1% Solution)	6.0 - 8.0
Endotoxin (LAL Test)	< 10 EU/mg (for applicable grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.