Description

2'-Chloro-2'-Deoxyuridine 5'-Triphosphate is a modified nucleotide triphosphate analog, a critical building block for molecular biology research and diagnostic applications. Its core value lies in its ability to act as a chain terminator or substrate analog in enzymatic reactions, enabling precise studies of nucleic acid structure and function. This compound is essential for researchers and manufacturers in the fields of molecular biology, biochemistry, and in vitro diagnostics, particularly for DNA sequencing, PCR-based assays, and the study of viral polymerases.

Application

• Molecular Biology Research: Used as a substrate or chain-terminating nucleotide in enzymatic studies of DNA and RNA polymerases.
• DNA Sequencing: Serves as a modified dNTP in Sanger sequencing and next-generation sequencing (NGS) methodologies.
• Polymerase Chain Reaction (PCR): Employed in specialized PCR applications to study polymerase fidelity or to incorporate specific modifications.
• Biochemical Assay Development: A key reagent for developing high-throughput screening assays targeting viral reverse transcriptases or other nucleic acid enzymes.
• Antiviral Research: Investigated for its potential to inhibit viral replication by acting as an alternative substrate for viral polymerases.
• Diagnostic Kit Manufacturing: Incorporated as a critical raw material in the production of kits for genetic testing and pathogen detection.

Basic Information

Product Name	2'-Chloro-2'-Deoxyuridine 5'-Triphosphate
CAS No.	61468-91-5
Molecular Formula	C9H14ClN2O14P3
Molecular Weight	526.60 g/mol
Synonyms	2'-Chloro-2'-deoxyuridine-5'-triphosphate; 2'-Cl-dUTP; Chlorodeoxyuridine triphosphate; 2'-Chloro-2'-deoxyuridine 5'-(tetrahydrogen triphosphate); 5'-Triphosphoric acid, 2'-chloro-2'-deoxyuridine; dCTP analog (2'-Cl); 2'-Chloro-dUTP
EINECS	Contact for details

Quality Control

Every batch of 2'-Chloro-2'-Deoxyuridine 5'-Triphosphate is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency, meeting the stringent requirements of life science research. Our quality system ensures compliance with relevant standards for research-grade biochemicals. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the vial to warm to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
Biological Activity	Suitable for use as polymerase substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.