Description

6-(2,2,6,6,-Tetramethylpiperidine-1-Oxyl)-Adenosine Diphosphate is a nitroxide spin-labeled derivative of adenosine diphosphate (ADP), a critical nucleotide in cellular energy transfer and signaling. This compound is specifically engineered for advanced research applications, particularly in electron paramagnetic resonance (EPR) spectroscopy, where it serves as a powerful molecular probe. It is essential for scientists and research institutions studying enzyme kinetics, protein-nucleotide interactions, and conformational changes in biochemical systems.

Application

• EPR/Spin Labeling Studies: Acts as a site-directed spin label for investigating the structure, dynamics, and binding interactions of ATP/ADP-binding proteins and enzymes.
• Enzymology Research: Used as a substrate analog to probe the active sites and catalytic mechanisms of kinases, ATPases, and other nucleotide-dependent enzymes.
• Membrane Protein Analysis: Employed in studying nucleotide interactions with membrane-bound receptors and transporters using EPR techniques.
• Biophysical Chemistry: A key tool for measuring distances and conformational dynamics within large protein complexes and nucleic acid structures.
• Drug Discovery Screening: Utilized in high-throughput assays to identify and characterize inhibitors targeting nucleotide-binding pockets.
• Metabolic Pathway Investigation: Serves as a tracer to study energy metabolism and nucleotide turnover in complex biological systems.

Basic Information

Product Name	6-(2,2,6,6,-Tetramethylpiperidine-1-Oxyl)-Adenosine Diphosphate
CAS No.	61468-67-5
Molecular Formula	C20H33N6O14P2
Molecular Weight	634.46 g/mol
Synonyms	6-TEMPO-ADP; TEMPO-Adenosine Diphosphate; Spin-Labeled ADP; 6-(2,2,6,6-Tetramethyl-1-oxyl-4-piperidinyl)adenosine 5'-diphosphate; Adenosine 5'-(trihydrogen diphosphate), 6-[4-(2,2,6,6-tetramethyl-1-oxyl-4-piperidinyl)]-; ADP-TEMPO; Nitroxyl-ADP; SL-ADP
EINECS	Contact for details

Quality Control

Every batch of 6-(2,2,6,6,-Tetramethylpiperidine-1-Oxyl)-Adenosine Diphosphate is manufactured and tested under strict quality management systems. We guarantee high purity and batch-to-batch consistency through comprehensive analytical techniques including HPLC, NMR, and EPR spectroscopy. A detailed Certificate of Analysis (COA) providing purity, identity, and stability data is supplied with each shipment to ensure it meets the exacting standards of research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the primary container sealed in a secondary, moisture-proof barrier. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (EPR)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.