Description

5-Ethynyl-2'-Deoxyuridine is a modified nucleoside analog that serves as a critical building block in chemical biology and pharmaceutical research. Its unique ethynyl group enables bioorthogonal click chemistry, making it an invaluable tool for labeling and tracking DNA synthesis in living cells. This compound is essential for researchers and manufacturers in the fields of oncology research, antiviral drug development, and molecular diagnostics.

Application

• DNA Labeling & Imaging: Acts as a thymidine analog for the metabolic labeling of newly synthesized DNA, enabling visualization via click chemistry with fluorescent azides.
• Cell Proliferation Assays: Used to study cell cycle dynamics, proliferation rates, and the effects of chemotherapeutic agents in cancer research.
• Antiviral Research: Investigated as a potential prodrug or intermediate in the development of nucleoside-based antiviral therapies.
• Chemical Biology Probes: Serves as a key precursor for synthesizing advanced molecular probes to study DNA replication and repair mechanisms.
• Diagnostic Kit Development: Utilized in the formulation of kits for detecting and quantifying DNA synthesis in pathological samples.
• Academic & Pharmaceutical R&D: A fundamental reagent in basic biochemical research and early-stage drug discovery pipelines.

Basic Information

Product Name	5-Ethynyl-2'-Deoxyuridine
CAS No.	61135-33-9
Molecular Formula	C11H12N2O5
Molecular Weight	252.22 g/mol
Synonyms	5-EdU; EdU; 5-Ethynyl-deoxyuridine; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-ethynyl-pyrimidine-2,4-dione; 2'-Deoxy-5-ethynyluridine; 5-Ethynyl-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl]pyrimidine-2,4(1H,3H)-dione
EINECS	Contact for details

Quality Control

Our 5-Ethynyl-2'-Deoxyuridine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards, and custom purity grades (e.g., >98% HPLC) are available to meet specific research or cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.