Description

4-Hydroxyestriol is a hydroxylated metabolite of the endogenous estrogen, estriol. This compound is of significant interest in biochemical and pharmaceutical research due to its role in estrogen metabolism and its potential as a reference standard. It serves as a critical intermediate and analytical standard for researchers and manufacturers in the life sciences sector, particularly those focused on endocrinology, steroid chemistry, and the development of diagnostic assays.

Application

• Pharmaceutical Reference Standard: Used as a high-purity certified reference material (CRM) for quality control and method validation in drug development and manufacturing.
• Biochemical Research: A vital tool for studying estrogen metabolism pathways, enzyme activity (e.g., cytochrome P450), and hormonal regulation in metabolic studies.
• Diagnostic Kit Development: Employed in the calibration and development of immunoassays and LC-MS/MS methods for the quantitative analysis of estrogen metabolites in clinical and research settings.
• Metabolite Synthesis: Acts as a key intermediate or precursor in the organic synthesis of more complex steroid derivatives and labeled compounds for tracer studies.
• Academic & Institutional Research: Supports fundamental research in universities and institutes exploring endocrinology, cancer research, and reproductive health.

Basic Information

Product Name	4-Hydroxyestriol
CAS No.	60021-32-1
Molecular Formula	C18H24O4
Molecular Weight	304.38 g/mol
Synonyms	Estra-1,3,5(10)-triene-3,16α,17β-triol; 16α-Hydroxyestriol; 1,3,5(10)-Estratrien-3,16α,17β-triol; 4-Hydroxyestriol; 4-OH-Estriol; 16α-Hydroxy-17β-estradiol
EINECS	Contact for details

Quality Control

Our 4-Hydroxyestriol is manufactured and handled under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and development. A detailed Certificate of Analysis (COA) specifying batch-specific results is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be kept under inert atmosphere or desiccated after opening to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Single Unknown Impurity (HPLC)	≤ 0.5%
Total Impurities (HPLC)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.