Description

Fluorescein Chloride is a vital reactive derivative of fluorescein, serving as a key intermediate for the synthesis of fluorescent probes and labels. Its primary value lies in its ability to covalently bind to amino groups, enabling the tagging of proteins, antibodies, and other biomolecules for detection and analysis. This compound is essential for researchers and manufacturers in the biotechnology, diagnostics, and pharmaceutical development sectors who require high-purity, reliable conjugation reagents.

Application

• Fluorescent Labeling Agent: Primary use for covalently labeling proteins, peptides, and antibodies via reaction with primary amines.
• Bioconjugation Intermediate: Critical starting material for synthesizing more complex fluorescent dyes and active esters (e.g., FITC analogs).
• Diagnostic Reagent Synthesis: Used in the manufacture of reagents for immunofluorescence assays (IFA) and fluorescence-activated cell sorting (FACS).
• Biomedical Research: Enables tracking and visualization of biomolecules in cellular imaging, flow cytometry, and microscopy studies.
• Sensor Development: Building block for creating chemical sensors and probes that detect specific ions or biological activities.
• Material Science: Modification of surfaces and polymers to introduce fluorescent properties for tracking and analysis.

Basic Information

Product Name	Fluorescein Chloride
CAS No.	630-88-6
Molecular Formula	C21H11ClO5
Molecular Weight	378.76 g/mol
Synonyms	Fluorescein Carbonyl Chloride; Fluorescein-5(6)-Carbonyl Chloride; 5(6)-Carboxyfluorescein Chloride; 3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5(6)-carbonyl chloride; Fluorescein-5-isothiocyanate precursor; FITC precursor; 5-FITC precursor; Reactive Fluorescein Derivative
EINECS	211-149-7

Quality Control

Our Fluorescein Chloride is produced under strict quality management systems to ensure batch-to-batch consistency and high reactivity. Each lot undergoes comprehensive analytical testing, including HPLC for purity, IR for identification, and titration for active chloride content. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the stringent requirements for research and bioconjugation applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent hydrolysis and maintain reactivity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.