Description

Cytidine Triphosphate CAS NO 65-47-4 is a high-purity nucleotide essential for RNA synthesis and various enzymatic processes. This critical biochemical serves as a direct substrate for RNA polymerases and is vital for cellular energy transfer and signaling pathways. It is primarily required by biotechnology, pharmaceutical, and life science research industries for applications in molecular biology, diagnostics, and drug development.

Application

• Molecular Biology Research: Essential substrate for in vitro transcription (IVT) reactions to synthesize mRNA, RNA probes, and other RNA molecules.
• Pharmaceutical Manufacturing: Used in the enzymatic synthesis of oligonucleotides and as a critical raw material for mRNA-based therapeutics and vaccines.
• Diagnostic Assay Development: Employed in the production of labeled nucleotides for detection systems in PCR, sequencing, and microarray technologies.
• Biochemical Studies: Serves as a key reagent in enzymatic studies, kinase assays, and research into cellular metabolism and signal transduction.
• Cell Culture & Media Supplementation: Used in specialized cell culture media to support nucleotide metabolism in fast-growing or engineered cell lines.

Basic Information

Product Name	Cytidine Triphosphate
CAS No.	65-47-4
Molecular Formula	C9H16N3O14P3
Molecular Weight	503.14 g/mol
Synonyms	CTP; Cytidine 5'-triphosphate; 5'-CTP; Cytidine-5'-triphosphate; Cytidine 5'-(tetrahydrogen triphosphate); 5'-Cytidylic acid triphosphate; Cytidine triphosphoric acid; 1-(5-O-{[Hydroxy({[hydroxy(phosphonooxy)phosphoryl]oxy})phosphoryl]methyl}-β-D-ribofuranosyl)-4-imino-2(1H)-pyrimidinone
EINECS	200-609-2

Quality Control

Our Cytidine Triphosphate is manufactured under strict quality systems to ensure it meets the stringent requirements of pharmaceutical and research applications. Each batch is tested for identity, purity, and the absence of critical impurities like nucleoside monophosphates and other nucleotides. A comprehensive Certificate of Analysis (COA) is provided with every shipment, detailing specifications such as HPLC purity, endotoxin levels, and bioburden where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95% (Typical ≥ 98%)
Water Content (KF)	≤ 5.0%
pH (1% Solution)	2.0 - 4.0
Endotoxin (LAL)	< 10 EU/mg (for injectable grade)
Solubility	Soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.