Description

Methacholine Chloride CAS NO 62-51-1 is a synthetic cholinergic agonist and parasympathomimetic agent. It is a critical diagnostic tool in pulmonary function testing, primarily used to assess airway hyperresponsiveness in conditions like asthma. This compound is essential for pharmaceutical manufacturers, clinical research organizations, and diagnostic laboratories worldwide.

Application

• Bronchial Challenge Testing: The primary application is in methacholine challenge tests (MCT) to diagnose and evaluate asthma and airway hyperresponsiveness.
• Pharmaceutical Research: Used as a reference standard and active agent in preclinical studies investigating cholinergic pathways, respiratory physiology, and potential bronchodilators.
• Diagnostic Kit Manufacturing: A key component in standardized diagnostic kits and solutions used in pulmonary function laboratories and clinical settings.
• Pharmacological Studies: Employed in vitro and in vivo to study muscarinic acetylcholine receptor (mAChR) activity, smooth muscle contraction, and autonomic nervous system responses.
• Quality Control & Calibration: Serves as a high-purity standard for the calibration of analytical equipment and validation of testing protocols in regulated environments.

Basic Information

Product Name	Methacholine Chloride
CAS No.	62-51-1
Molecular Formula	C8H18ClNO2
Molecular Weight	195.69 g/mol
Synonyms	Acetyl-β-methylcholine chloride; (2-Hydroxypropyl)trimethylammonium chloride acetate; Mecholyl chloride; Provocholine®; β-Methylacetylcholine chloride; Amechol; Methacholine; O-Acetyl-β-methylcholine chloride
EINECS	200-536-5

Quality Control

Our Methacholine Chloride is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing including identification, assay, and impurity profiling to ensure it meets exacting standards for diagnostic and research use. Certificates of Analysis (COA) with full traceability are provided, confirming compliance with relevant specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept in a dry environment to maintain stability and potency.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Related Substances (HPLC)	Individual impurity ≤ 0.5%; Total impurities ≤ 1.5%
Microbial Limits	Meets requirements for absence of specified microorganisms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.