Description

5'-Dmt-2'-Tbdms-N6-Me-Ra CAS NO 588698-75-3 is a high-purity, protected nucleoside phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. This compound is essential for researchers and manufacturers requiring precise control over nucleic acid sequence and modification, enabling the development of advanced therapeutics and diagnostic tools. It is primarily utilized by biotechnology and pharmaceutical companies engaged in the production of antisense oligonucleotides, siRNAs, aptamers, and other novel nucleic acid-based entities.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of modified RNA strands.
• Therapeutic Development: For the research and production of antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) for targeted gene silencing.
• Diagnostic Probe Manufacture: In creating labeled and modified nucleic acid probes for molecular diagnostics and hybridization assays.
• Aptamer Production: For synthesizing chemically modified RNA aptamers with enhanced stability and binding affinity.
• Basic Research: Enabling academic and industrial research into RNA structure, function, and RNA-protein interactions.
• CRISPR/Cas System Components: Potential use in synthesizing modified guide RNAs (gRNAs) for genome editing applications.

Basic Information

Product Name	5'-Dmt-2'-Tbdms-N6-Me-Ra
CAS No.	588698-75-3
Molecular Formula	C46H64N7O7P Si
Molecular Weight	886.11 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N6-methyladenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; N6-Methyl-2'-O-TBDMS-5'-O-DMT-adenosine-3'-CE-phosphoramidite; 2'-O-TBDMS-N6-Me-Adenosine Phosphoramidite; N6-Methyladenosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[[(1,1-dimethylethyl)dimethylsilyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 6-Methylamino-9-[(2R,3R,4R,5R)-5-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-3-[[tert-butyl(dimethyl)silyl]oxy]-4-hydroxyoxolan-2-yl]purine 3-(2-cyanoethoxy)diisopropylaminophosphine
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Quality Control

Our 5'-Dmt-2'-Tbdms-N6-Me-Ra is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and Mass Spectrometry, to ensure it meets the stringent requirements for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee consistency, purity (>98% typical), and performance in your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.