Description

N3-Peg4-Tbu CAS NO 581066-04-8 is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide (N3) group and a tert-butyl (tBu) protected carboxylic acid, separated by a tetraethylene glycol (PEG4) spacer. This compound is essential for creating stable, biocompatible conjugates in bioconjugation and click chemistry applications, where controlled linkage and solubility are critical. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and advanced materials sectors for developing drug delivery systems, diagnostic probes, and functionalized surfaces.

Application

• Bioconjugation and Linker Chemistry: Serves as a versatile heterobifunctional crosslinker for coupling biomolecules like proteins, peptides, and oligonucleotides via copper-free or copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry.
• ADC (Antibody-Drug Conjugate) Development: Used to construct stable, cleavable linkers between monoclonal antibodies and cytotoxic payloads, leveraging the PEG spacer for improved solubility and pharmacokinetics.
• Polymer and Surface Functionalization: Enables the modification of polymers, nanoparticles, and solid surfaces with bioactive ligands or reporter molecules for targeted delivery and sensing applications.
• Proteomics and Chemical Biology: Acts as a tool compound for labeling and enriching biomolecules in activity-based protein profiling (ABPP) and other discovery research platforms.
• Diagnostic Reagent Synthesis: Employed in the synthesis of contrast agents, fluorescent probes, and other diagnostic reagents where a hydrophilic PEG spacer is beneficial.
• Hydrogel and Biomaterial Fabrication: Functions as a crosslinking agent in the development of PEG-based hydrogels for tissue engineering and controlled release matrices.

Basic Information

Product Name	N3-Peg4-Tbu
CAS No.	581066-04-8
Molecular Formula	C19H35N3O7
Molecular Weight	417.50 g/mol
Synonyms	Azido-PEG4-t-butyl ester; 2,5,8,11-Tetraoxatetradecan-14-oic acid, 13-azido-4-oxo-3,6,9,12-tetraoxa-15-azaoctadec-17-en-18-yl ester, tert-butyl; 1-Azido-3,6,9,12-tetraoxapentadecan-15-oic acid tert-butyl ester; N3-PEG4-COOtBu; tert-Butyl 17-azido-4,7,10,13-tetraoxa-16-azaheptadecanoate; Azido-PEG4-acid t-butyl ester; PEG4 azide t-butyl ester; Azido tetraethylene glycol tert-butyl ester
EINECS	Contact for details

Quality Control

Our N3-Peg4-Tbu is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and MS for identity verification, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or CoA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Colorless to pale yellow oil or viscous liquid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.