Description

N2-Acetyl-5'-o-(4,4'-Dimethoxytrityl)-2'-Deoxy-2'-Fluoroguanosine is a high-purity, protected nucleoside derivative essential for oligonucleotide synthesis. This compound serves as a critical building block for the solid-phase assembly of modified RNA and DNA strands, enabling advanced research and therapeutic development. It is primarily required by pharmaceutical R&D laboratories, biotechnology companies, and CROs specializing in antisense oligonucleotides, siRNA, and aptamer development.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Key phosphoramidite precursor for automated synthesis of 2'-fluoro-modified oligonucleotides.
• Antisense & RNAi Therapeutics: Building block for creating nuclease-resistant therapeutic oligonucleotides with enhanced stability and binding affinity.
• Diagnostic Probe Development: Used in the manufacture of fluorescently labeled or otherwise tagged probes for molecular diagnostics and FISH assays.
• Aptamer Research: Enables the synthesis of chemically modified SELEX libraries to discover high-affinity aptamers for therapeutic and diagnostic use.
• Structural Biology Studies: Incorporation into RNA/DNA strands for NMR and X-ray crystallography to study nucleic acid structure and function.
• Process Development & Scale-Up: Serves as a starting material for GMP-grade production of active pharmaceutical ingredients (APIs) for oligonucleotide drugs.

Basic Information

Product Name	N2-Acetyl-5'-o-(4,4'-Dimethoxytrityl)-2'-Deoxy-2'-Fluoroguanosine
CAS No.	514830-14-9
Molecular Formula	C33H32FN5O7
Molecular Weight	629.64 g/mol
Synonyms	2'-Deoxy-2'-fluoro-N2-acetyl-5'-O-(4,4'-dimethoxytrityl)guanosine; 5'-O-DMT-2'-fluoro-N2-acetyl-2'-deoxyguanosine; 2'-F-dG(N-Ac)-5'-DMT; 2'-Fluoro-2'-deoxy-N2-acetyl-5'-O-DMT-guanosine; Guanosine, 2'-deoxy-2'-fluoro-N2-acetyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; DMT-2'F-dG(Ac) Amidite Precursor; N2-Ac-5'-O-DMT-2'-F-dG
EINECS	Contact for details

Quality Control

Our N2-Acetyl-5'-o-(4,4'-Dimethoxytrityl)-2'-Deoxy-2'-Fluoroguanosine is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive synthetic applications. Each lot undergoes rigorous analytical testing, including HPLC purity analysis, NMR structural confirmation, and mass spectrometry. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.