Description

5'-O-(4,4'-Dimethoxytrityl)-N2-Dimethylaminomethylene-8-Aza-7-Deaza-2'-Deoxyguanosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is a high-purity, modified nucleoside phosphoramidite monomer essential for solid-phase oligonucleotide synthesis. This compound is specifically engineered to introduce a 7-deaza-8-aza-guanine base analog into DNA sequences, which is critical for applications requiring enhanced duplex stability and resistance to nuclease degradation. It is a vital raw material for research institutions, diagnostic developers, and pharmaceutical companies engaged in the production of antisense oligonucleotides, primers, probes, and therapeutic nucleic acids.

Application

• Synthesis of Modified Oligonucleotides: Key monomer for incorporating 8-aza-7-deaza-2'-deoxyguanosine analogs into DNA strands.
• Therapeutic Nucleic Acid Development: Used in the research and GMP-scale production of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug discovery.
• Diagnostic Probe Manufacturing: Essential for creating high-affinity, nuclease-resistant probes for PCR, FISH, and microarray technologies.
• Structural Biology Studies: Enables the study of DNA-protein interactions and duplex stability by modifying base-pairing properties.
• Next-Generation Sequencing (NGS): Used in the synthesis of specialized primers and adapters for advanced sequencing platforms.
• Synthetic Biology: Building block for constructing artificial genes and genetic circuits with tailored physicochemical properties.

Basic Information

Item	Detail
Product Name	5'-O-(4,4'-Dimethoxytrityl)-N2-Dimethylaminomethylene-8-Aza-7-Deaza-2'-Deoxyguanosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite
CAS No.	500891-26-9
Molecular Formula	C46H55N8O7P
Molecular Weight	863.0 g/mol
Synonyms	8-Aza-7-deaza-dG Phosphoramidite; c7-GdA Phosphoramidite; Pyrrolo[2,3-d]pyrimidine-2-one phosphoramidite; DMT-8-Aza-7-Deaza-dG-CE Phosphoramidite; 5'-O-DMT-N2-DMF-8-aza-7-deaza-2'-deoxyguanosine-3'-CE phosphoramidite; 8-Aza-7-deaza-2'-deoxyguanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2-[[(dimethylamino)methylene]amino]-3-[(2-cyanoethoxy)(diisopropylamino)phosphinyl]-; c7G(dmf) Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N2-Dimethylaminomethylene-8-Aza-7-Deaza-2'-Deoxyguanosine Phosphoramidite is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC purity analysis, 31P NMR, and MS confirmation, to ensure it meets the high standards required for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment, supporting compliance with ISO and GMP guidelines for advanced pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, freezer environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.