Description

2-Thiopseudouridine is a modified nucleoside analog of pseudouridine, where the oxygen atom in the uracil ring is replaced by a sulfur atom at the 2-position. This structural modification is of significant interest in the development of novel therapeutic agents and research tools, particularly in the fields of antiviral and anticancer drug discovery. It serves as a critical building block for pharmaceutical research and development, especially for scientists working on RNA-based therapeutics, nucleoside chemistry, and medicinal chemistry.

Application

• Pharmaceutical Intermediate: A key synthetic precursor for the development of novel antiviral and anticancer nucleoside analogs.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies to investigate RNA modification pathways and pseudouridine synthases.
• Medicinal Chemistry: Serves as a core scaffold for structure-activity relationship (SAR) studies to optimize drug potency and selectivity.
• RNA Therapeutics: Investigated for its potential to enhance the stability and efficacy of synthetic mRNA and other oligonucleotide-based therapies.
• Chemical Biology Probe: Utilized to study the function and localization of pseudouridine in cellular RNA through metabolic labeling or affinity tagging strategies.
• Reference Standard: Employed as an analytical standard in quality control laboratories for the identification and quantification of related compounds.

Basic Information

Product Name	2-Thiopseudouridine
CAS No.	59464-18-5
Molecular Formula	C9H12N2O5S
Molecular Weight	260.27 g/mol
Synonyms	2-Thio-ψ-uridine; 2-Thio-5-β-D-ribofuranosyluracil; 2-Thio-5-ribosyluracil; 2-Thiouridine, 5-ribosyl-; Pseudouridine, 2-thio-; 5-(β-D-Ribofuranosyl)-2-thiouracil; 2-Thio-5-ribofuranosyluracil
EINECS	Contact for details

Quality Control

Our 2-Thiopseudouridine is produced under a strict quality management system. Every batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and tests for residual solvents and moisture content. A Certificate of Analysis (COA) detailing all specifications and results is provided with each shipment to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which may affect stability and handling.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.