Description

8-Azidoadenosine Diphosphate (8-N3-ADP) is a chemically modified nucleotide analog featuring a photoreactive azido group, making it a critical tool for photoaffinity labeling and crosslinking studies in biochemistry. This compound is essential for researchers investigating nucleotide-binding sites, enzyme mechanisms, and protein-protein interactions with high specificity. It is primarily utilized in academic research, pharmaceutical R&D, and biotechnology for probing ATP-binding proteins, kinases, and other nucleotide-dependent enzymes.

Application

• Photoaffinity Labeling Probe: Used to covalently tag and identify ATP/ADP binding sites on proteins and enzymes upon UV irradiation.
• Enzyme Mechanism Studies: Employed as a substrate analog to investigate the catalytic mechanisms of kinases, synthetases, and other nucleotide-processing enzymes.
• Structural Biology: Aids in mapping binding pockets and conformational changes in proteins through crosslinking experiments for structural analysis.
• Drug Discovery Target Validation: Serves as a tool compound to validate and characterize novel drug targets that interact with adenosine phosphates.
• Bioconjugation and Probe Synthesis: Acts as a building block for synthesizing more complex biochemical probes via click chemistry (e.g., with alkynes).
• Metabolic Pathway Analysis: Used to trace and study metabolic pathways involving nucleotide derivatives in cellular systems.

Basic Information

Item	Detail
Product Name	8-Azidoadenosine Diphosphate
CAS No.	59432-65-4
Molecular Formula	C10H14N8O10P2
Molecular Weight	468.18 g/mol
Synonyms	8-N3-ADP; 8-Azido-ADP; Adenosine 5'-(trihydrogen diphosphate), 8-azido-; 8-Azidoadenosine 5'-diphosphate; 8-Azido-adenosine-5'-diphosphate; 8-Azido-5'-ADP; 8-Azidoadenosine pyrophosphate
EINECS	Contact for details

Quality Control

Our 8-Azidoadenosine Diphosphate is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality standards for research chemicals, ensuring reliable performance in your experimental workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, light, or ambient temperatures will lead to decomposition. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water or buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.