Description

N4-Desmethyl-N5-Methyl Wyosine CAS NO 59327-63-8 is a modified nucleoside derivative, specifically a hypermodified guanosine analog found in transfer RNA (tRNA). This compound is of significant interest in biochemical and pharmaceutical research due to its role in tRNA structure and function, which influences translational fidelity and efficiency. It serves as a critical reference standard and building block for researchers in nucleic acid chemistry, molecular biology, and drug discovery, particularly for studies involving tRNA modification enzymes and the development of novel therapeutics.

Application

• Biochemical Research Standard: Used as a high-purity reference compound for the identification and quantification of modified nucleosides in tRNA analysis.
• Enzymology Studies: Serves as a substrate or inhibitor in assays for tRNA-modifying enzymes, such as methyltransferases.
• Nucleic Acid Chemistry: Acts as a key intermediate or precursor in the synthetic chemistry of modified oligonucleotides for structural and functional studies.
• Pharmaceutical Development: Investigated for its potential role in targeting translation-related pathways for therapeutic intervention.
• Diagnostic Tool Development: Potential use in developing assays to detect specific tRNA modifications linked to disease states.
• Academic Research: Essential for fundamental studies in molecular genetics, epigenetics, and post-transcriptional gene regulation.

Basic Information

Product Name	N4-Desmethyl-N5-Methyl Wyosine
CAS No.	59327-63-8
Molecular Formula	C17H23N5O6
Molecular Weight	393.40 g/mol
Synonyms	N(4)-Demethyl-N(5)-methylwyosine; 4-Demethyl-5-methylwyosine; 1-Methyl-4-demethylwyosine; 2-Amino-5,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one derivative; tRNA nucleoside modification; Hypermodified guanosine; Wybutosine-related nucleoside
EINECS	Contact for details

Quality Control

Our N4-Desmethyl-N5-Methyl Wyosine is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and residual solvent analysis. We provide Certificates of Analysis (COA) with each shipment, detailing all test results to ensure the material meets the stringent requirements for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain purity. Allow the container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.