Description

5-Azidomethyl-2'-Deoxyuridine is a chemically modified nucleoside analog that serves as a critical building block in nucleic acid research and development. This compound is valued for its unique azido functional group, which enables bioorthogonal click chemistry for labeling and cross-linking applications. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, molecular biology, and diagnostic probe synthesis.

Application

• Nucleic Acid Labeling and Sequencing: Acts as a key reagent for the site-specific incorporation of clickable handles into DNA strands for next-generation sequencing and fluorescent tagging.
• Antiviral and Anticancer Drug Research: Serves as a precursor or intermediate in the synthesis of novel nucleoside-based prodrugs targeting viral polymerases and cellular replication pathways.
• Bioconjugation and Probe Development: Used to create cross-linked nucleic acid-protein complexes or to attach affinity tags (e.g., biotin, fluorophores) via copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• Structural Biology Studies: Incorporated into oligonucleotides to study DNA-protein interactions, enzyme mechanisms, and nucleic acid structure through photoaffinity labeling.
• Diagnostic Assay Kits: Employed in the manufacturing of specialized probes for PCR-based diagnostics, FISH assays, and other molecular detection systems.

Basic Information

Product Name	5-Azidomethyl-2'-Deoxyuridine
CAS No.	59090-48-1
Molecular Formula	C10H11N5O5
Molecular Weight	281.23 g/mol
Synonyms	5-(Azidomethyl)-2'-deoxyuridine; 5-Azidomethyl-deoxyuridine; 5-(Azidomethyl)-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione; 5-N3MdU; Azidomethyl dU; 5-Azidomethyl-dU
EINECS	Contact for details

Quality Control

Every batch of 5-Azidomethyl-2'-Deoxyuridine is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing specifications such as HPLC purity, NMR confirmation, and residual solvent analysis. Our quality system is designed to meet the stringent requirements of research and pharmaceutical intermediate applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. The compound is light-sensitive and should be handled under appropriate conditions to prevent degradation. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.