Description

2'-o-Methylcytidine is a chemically modified nucleoside derivative where a methyl group is attached to the 2' oxygen of the ribose sugar. This specific modification is crucial for enhancing the stability and nuclease resistance of oligonucleotides, making it a vital building block in advanced therapeutic and research applications. It is primarily utilized by biotechnology, pharmaceutical, and academic research sectors engaged in the development of antisense oligonucleotides, siRNA, and other RNA-based therapeutics.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A key raw material for constructing stable, nuclease-resistant antisense drugs targeting specific mRNA sequences.
• Small Interfering RNA (siRNA) Production: Used to introduce 2'-O-methyl modifications, improving siRNA stability and pharmacokinetic properties for gene silencing applications.
• Therapeutic Aptamer Development: Serves as a modified nucleotide in creating stable nucleic acid aptamers for targeted drug delivery and diagnostics.
• Biochemical Research: Employed as a critical reagent in studies investigating RNA structure, function, and RNA-protein interactions.
• Diagnostic Probe Fabrication: Used in the synthesis of modified probes for sensitive and stable detection of nucleic acids in diagnostic assays.
• Pharmaceutical Intermediates: Acts as a high-purity intermediate in the multi-step synthesis of complex nucleoside-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	2'-o-Methylcytidine
CAS No.	58970-16-4
Molecular Formula	C10H15N3O5
Molecular Weight	257.24 g/mol
Synonyms	2'-O-Methylcytidine; 1-[(2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]-4-aminopyrimidin-2-one; Cytidine, 2'-O-methyl-; 2'-OMe-C; 2'-O-Methylcytidine (C10H15N3O5); 4-Amino-1-[(2R,3R,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]pyrimidin-2-one; 2'-Methylcytidine
EINECS	Contact for details

Quality Control

Our 2'-o-Methylcytidine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture to ensure it meets the high standards required for pharmaceutical and biotech applications. A comprehensive Certificate of Analysis (COA) is provided with every shipment, detailing all specification results.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.