Description

5-Hydroxymethyl-2-Thiouridine is a modified nucleoside derivative of uridine, where the 5-position is hydroxymethylated and the 2-oxo group is replaced with a thione. This compound is a critical building block and research chemical in the fields of nucleic acid chemistry and molecular biology. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotechnology sectors for the synthesis of modified oligonucleotides, enabling the study of RNA structure, function, and stability, as well as the development of novel therapeutic agents.

Application

• Oligonucleotide Synthesis: A key monomer for the solid-phase synthesis of thio-modified RNA strands to study structure-function relationships.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies to investigate RNA metabolism and processing.
• Antiviral/Anticancer Drug Development: Serves as a precursor in the research and development of nucleoside analog prodrugs targeting viral polymerases or cellular replication.
• RNA Aptamer Development: Incorporated into SELEX libraries to generate modified RNA aptamers with enhanced nuclease resistance and binding affinity.
• Diagnostic Probe Design: Used in the creation of labeled probes for detecting specific RNA sequences, where the thio-modification can alter hybridization properties.
• Academic Research: Fundamental studies on the effect of sugar and base modifications on duplex stability, codon recognition, and translational fidelity.

Basic Information

Product Name	5-Hydroxymethyl-2-Thiouridine
CAS No.	58479-79-1
Molecular Formula	C10H14N2O6S
Molecular Weight	290.29 g/mol
Synonyms	2-Thio-5-hydroxymethyluridine; 5-(Hydroxymethyl)-2-thiouridine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-(hydroxymethyl)-2-thioxo-1,2-dihydropyrimidin-4(3H)-one; 5-Hydroxymethyl-2-thio-uridine; 2-Thiouridine, 5-(hydroxymethyl)-; s2Uhm; 5-HMTU
EINECS	Contact for details

Quality Control

Our 5-Hydroxymethyl-2-Thiouridine is produced and tested under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry guidelines to support our customers' quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. Due to its hygroscopic nature, the container should be kept sealed in a desiccated environment to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥95.0%
Water Content (KF)	≤5.0%
Related Substances (HPLC)	Total impurities ≤3.0%
Solubility	Soluble in DMSO, sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.