Description

7-Methylguanosine-5'-Triphosphoryl-2'-o-Methylguanosine is a chemically modified dinucleotide cap analog, a critical reagent in molecular biology and biochemistry. This compound is essential for the in vitro synthesis of capped mRNA transcripts, mimicking the natural 5' cap structure to enhance stability and translational efficiency. It is primarily utilized by researchers and manufacturers in pharmaceutical development, biotechnology, and life sciences, particularly for mRNA vaccine and therapeutic production, as well as fundamental studies in gene expression and protein synthesis.

Application

• mRNA Synthesis & Capping: As a high-efficiency cap analog for in vitro transcription to produce capped mRNA for therapeutic and vaccine applications.
• Biochemical Research: Used in studies of translation initiation, mRNA metabolism, and cap-dependent cellular processes.
• Pharmaceutical Development: A key raw material in the manufacturing process of mRNA-based vaccines and gene therapies.
• Diagnostic Probe Synthesis: For creating labeled or modified mRNA probes used in hybridization and detection assays.
• Enzymology Studies: Serving as a substrate for enzymes involved in mRNA capping and decapping pathways.

Basic Information

Product Name	7-Methylguanosine-5'-Triphosphoryl-2'-o-Methylguanosine
CAS No.	57718-00-0
Molecular Formula	C23H31N10O17P3
Molecular Weight	836.47 g/mol
Synonyms	m7G(5')ppp(5')Gm; Cap 0 Analog (m7GpppGm); 7-Methyl-GpppG; Anti-Reverse Cap Analog (ARCA) variant; 2'-O-Methyl-m7GpppG; mRNA Cap Analog; Dinucleotide Cap 0; N7-Methylguanosine-5'-triphosphoryl-2'-O-methylguanosine
EINECS	Contact for details

Quality Control

Our 7-Methylguanosine-5'-Triphosphoryl-2'-o-Methylguanosine is produced under stringent conditions suitable for research and biopharmaceutical applications. Quality is assured through comprehensive analytical testing, including HPLC for purity and identity confirmation. We provide full traceability and Certificates of Analysis (COA) with each batch, detailing purity, assay, and impurity profiles to support your regulatory and quality requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by UV)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or appropriate buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.