Description

3β-Hydroxy-5-Cholestenoic Acid is a high-purity steroidal acid derivative, serving as a critical intermediate and reference standard in advanced biochemical research and pharmaceutical development. Its value lies in its role as a key metabolite in the study of cholesterol biosynthesis and bile acid pathways, enabling precise analytical and synthetic applications. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable, high-quality biochemical building blocks.

Application

• Pharmaceutical Intermediate: A key synthetic precursor in the development of novel steroidal drugs and therapeutic agents.
• Biochemical Research: Used as a reference standard and metabolite in studies of cholesterol metabolism, liver function, and bile acid synthesis pathways.
• Diagnostic Reagent Manufacturing: Employed in the production of calibrators and controls for clinical diagnostic assays targeting sterol and bile acid levels.
• Metabolomics & Lipidomics: Serves as an important analytical standard in mass spectrometry-based profiling of sterol-related metabolites.
• Academic & Contract Research: Utilized in university and CRO laboratories for fundamental research into lipid biochemistry and enzymology.

Basic Information

Product Name	3β-Hydroxy-5-Cholestenoic Acid
CAS No.	56845-87-5
Molecular Formula	C27H44O3
Molecular Weight	416.64 g/mol
Synonyms	3β-Hydroxy-5-cholestenoic acid; (3β)-3-Hydroxycholest-5-en-26-oic Acid; 5-Cholestene-3β,26-diol-26-oic Acid; 26-Carboxy-26-norcholesterol; 3β-Hydroxy-5-cholesten-26-oic Acid; NSC 6135
EINECS	Contact for details

Quality Control

Our 3β-Hydroxy-5-Cholestenoic Acid is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research and development. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions should be avoided to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.