Description

4-Thiouridine 2',3',5'-Triacetate is a chemically modified nucleoside derivative where the ribose sugar is protected with acetyl groups and the uracil base contains a sulfur substitution at the 4-position. This compound is a crucial intermediate in oligonucleotide synthesis and chemical biology research, enabling the site-specific introduction of thiol functionality into nucleic acid chains. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for developing modified RNA, cross-linking studies, and creating affinity probes.

Application

• Oligonucleotide Synthesis: A key building block for the solid-phase synthesis of thiol-modified RNA strands for structural and functional studies.
• Bioconjugation & Probe Development: The thiol group allows for specific conjugation with maleimides or other electrophiles, enabling the creation of fluorescent, affinity, or therapeutic probes.
• Cross-linking & Structural Biology: Used to investigate RNA-protein interactions or RNA structure through UV-induced cross-linking via the thione group.
• Antisense & Therapeutic RNA Research: Serves as a precursor for incorporating modifications that can enhance the stability, delivery, or targeting of therapeutic oligonucleotides.
• Chemical Biology Tool Development: Employed in the synthesis of novel nucleoside analogs for screening and mechanistic studies.

Basic Information

Product Name	4-Thiouridine 2',3',5'-Triacetate
CAS No.	55003-25-3
Molecular Formula	C16H18N2O9S
Molecular Weight	414.39 g/mol
Synonyms	4-Thio-uridine 2',3',5'-triacetate; 2',3',5'-Tri-O-acetyl-4-thiouridine; 4-Thiouridine triacetate; 1-[(2R,3R,4R,5R)-3,4-Diacetyloxy-5-(acetyloxymethyl)oxolan-2-yl]-4-sulfanylidene-1,2,3,4-tetrahydropyrimidin-2-one; s4U triacetate; 4-thio-U triacetate
EINECS	Contact for details

Quality Control

Our 4-Thiouridine 2',3',5'-Triacetate is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.