Description

S-(5''-Adenosyl)-D-Homocysteine is a high-purity biochemical compound, also known as S-Adenosyl-D-homocysteine (D-SAH). This molecule serves as a key metabolite and a potent, non-reactive inhibitor of S-adenosyl-L-methionine (SAM)-dependent methyltransferases, making it an essential tool for epigenetic and biochemical research. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and diagnostic manufacturers. Its high purity and stability are critical for ensuring reliable and reproducible experimental results in enzymology and cellular studies.

Application

• Biochemical Research: As a standard inhibitor in studies of transmethylation reactions, protein arginine methyltransferases (PRMTs), DNA methyltransferases (DNMTs), and histone methyltransferases.
• Pharmaceutical Development: Used in high-throughput screening (HTS) assays to identify and characterize novel therapeutic agents targeting epigenetic regulation.
• Enzymology Studies: Serves as a critical substrate analog for investigating the kinetics and mechanisms of SAM-dependent methyltransferase enzymes.
• Diagnostic Reagents: A component in enzymatic assay kits for clinical research and the measurement of methyltransferase activity.
• Cell Culture & Molecular Biology: Applied in cell-based assays to modulate cellular methylation status and study downstream effects on gene expression and cell function.

Basic Information

Product Name	S-(5''-Adenosyl)-D-Homocysteine
CAS No.	53276-26-9
Molecular Formula	C14H20N6O5S
Molecular Weight	384.41 g/mol
Synonyms	S-Adenosyl-D-homocysteine; D-SAH; D-S-Adenosylhomocysteine; AdoHcy-D; (2R)-2-Amino-4-({[(2S,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl}sulfanyl)butanoic acid; SAH-D; S-(5'-Adenosyl)-D-homocysteine; Adenosine, 5'-S-[(2R)-2-amino-3-carboxypropyl]-5'-thio-, (S)-
EINECS	Contact for details

Quality Control

Our S-(5''-Adenosyl)-D-Homocysteine is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for optical rotation to ensure enantiomeric purity. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming compliance with our stringent specifications for research and pharmaceutical-grade materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Optical Rotation	[α]²⁰/D: Specific range provided on COA
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.