Description

3'-tert-Butyldimethylsilyl-2'-Deoxycytidine is a key protected nucleoside derivative, widely used as a critical building block in oligonucleotide synthesis. Its primary value lies in providing a robust and selective protecting group for the 3'-hydroxyl position, enabling the controlled assembly of DNA sequences. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors, particularly for the development of antisense oligonucleotides, primers, and probes.

Application

• Oligonucleotide Synthesis: A vital phosphoramidite precursor for solid-phase and solution-phase synthesis of DNA strands.
• Antisense Therapy Development: Used in the research and production of antisense oligonucleotides for targeted gene silencing.
• Diagnostic Probe Manufacture: Serves as a building block for labeled probes used in PCR, sequencing, and microarray technologies.
• Pharmaceutical Intermediates: An important intermediate in the synthesis of nucleoside-based antiviral and anticancer drug candidates.
• Biochemical Research: Employed in academic and industrial R&D for studying nucleic acid chemistry, enzyme mechanisms, and molecular biology.
• CRISPR/Cas9 Component Synthesis: Used in the preparation of guide RNAs and other synthetic nucleic acid components for gene-editing applications.

Basic Information

Product Name	3'-tert-Butyldimethylsilyl-2'-Deoxycytidine
CAS No.	51549-28-1
Molecular Formula	C15H27N3O4Si
Molecular Weight	341.48 g/mol
Synonyms	3'-O-(tert-Butyldimethylsilyl)-2'-deoxycytidine; 3'-TBDMS-2'-Deoxycytidine; 2'-Deoxycytidine, 3'-(1,1-dimethylethyl)dimethylsilyl ether; 3'-O-(tert-Butyl(dimethyl)silyl)-2'-deoxycytidine; N4-Benzoyl-3'-O-(tert-butyldimethylsilyl)-2'-deoxycytidine (protected form); dC(TBDMS); TBDMS-dC; 3'-TBDMS-dC
EINECS	Contact for details

Quality Control

Our 3'-tert-Butyldimethylsilyl-2'-Deoxycytidine is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for sensitive synthetic applications. Certificates of Analysis (COA) detailing purity, moisture content, and related substance profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Keep away from strong bases and fluorides.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Related Substances (HPLC)	Individual impurity ≤0.5%; Total impurities ≤2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.