Description

5-Bromo-2'-Deoxy-5'-Uridylic Acid Disodium Salt is a modified nucleoside monophosphate derivative, serving as a critical building block in oligonucleotide synthesis and biochemical research. Its primary value lies in enabling the site-specific introduction of bromine atoms into nucleic acid sequences, which is essential for studying DNA-protein interactions, cross-linking experiments, and developing therapeutic oligonucleotides. This high-purity biochemical is indispensable for pharmaceutical R&D, academic laboratories, and biotechnology companies focused on molecular biology, antisense technology, and diagnostic probe development.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of modified DNA and RNA strands containing brominated uracil bases.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies involving polymerases, nucleotidyltransferases, and other nucleic acid-metabolizing enzymes.
• Photocrosslinking Studies: The bromine atom serves as a potential site for UV-induced crosslinking to probe nucleic acid structure and protein-DNA binding sites.
• Antisense & Therapeutic Development: Incorporated into antisense oligonucleotides (ASOs) or siRNA to modulate gene expression and develop novel therapeutics.
• Diagnostic Probe Fabrication: Used in the creation of labeled probes for techniques like fluorescence in situ hybridization (FISH) and microarray analysis.
• Structural Biology: Aids in X-ray crystallography and NMR studies by providing heavy atom derivatives for phasing in nucleic acid structure determination.

Basic Information

Product Name	5-Bromo-2'-Deoxy-5'-Uridylic Acid Disodium Salt
CAS No.	51432-32-7
Molecular Formula	C9H11BrN2Na2O8P
Molecular Weight	449.05 g/mol
Synonyms	5-Bromo-2'-deoxyuridine 5'-monophosphate disodium salt; 5-BrdUMP disodium salt; 5-Bromo-dUMP disodium salt; 5-Bromo-2'-deoxyuridylic acid disodium salt; BrdUMP Na2; 5-Bromodeoxyuridine monophosphate disodium; 5-Br-dUMP disodium
EINECS	Contact for details

Quality Control

Every batch of 5-Bromo-2'-Deoxy-5'-Uridylic Acid Disodium Salt is manufactured and tested under strict quality management systems. We provide comprehensive analytical documentation to ensure the material meets the stringent requirements for biochemical and pharmaceutical applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available for each lot upon request, supporting your regulatory and research needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 3.0%
pH (1% Solution)	6.5 - 8.5
Endotoxin	< 10 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.