Description

Thymidine, 4-o-Methyl- is a chemically modified nucleoside derivative, specifically a methylated analog of thymidine. This compound is of significant interest in biochemical and pharmaceutical research due to its role as a building block and a tool for studying nucleic acid metabolism and enzyme function. It is primarily utilized by research institutions and companies in the life sciences sector for applications in nucleotide synthesis, antiviral research, and as a reference standard.

Application

• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies, particularly for thymidine kinase and other nucleoside-metabolizing enzymes.
• Nucleotide Synthesis: Serves as a key intermediate or precursor in the chemical and enzymatic synthesis of modified oligonucleotides and nucleoside analogs.
• Antiviral & Anticancer Agent Development: Investigated for its potential incorporation into novel prodrugs or active pharmaceutical ingredients (APIs) targeting viral replication or cancer cell proliferation.
• Metabolic Pathway Studies: Employed as a tracer or probe to investigate nucleoside salvage pathways and DNA synthesis mechanisms in cellular models.
• Pharmaceutical Reference Standard: Used as a high-purity certified reference material (CRM) for quality control and analytical method development in drug manufacturing.

Basic Information

Product Name	Thymidine, 4-o-Methyl-
CAS No.	50591-13-4
Molecular Formula	C11H16N2O5
Molecular Weight	256.25 g/mol
Synonyms	4-O-Methylthymidine; 1-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-4-methoxy-5-methylpyrimidin-2(1H)-one; 5-Methyl-4-O-methyluridine; 4-Methoxythymidine; 4-O-Methyl-2'-deoxythymidine; 1-(2-Deoxy-β-D-erythro-pentofuranosyl)-4-methoxy-5-methyl-2(1H)-pyrimidinone
EINECS	Contact for details

Quality Control

Our Thymidine, 4-o-Methyl- is produced and tested under a strict quality management system. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods (NMR, IR, MS). Certificates of Analysis (COA) detailing batch-specific results are available upon request to ensure compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.