Description

4-Hydroxyestradiol is a significant endogenous metabolite of estradiol, playing a crucial role in estrogen metabolism and associated biological pathways. This compound is of high importance for research and development in the fields of endocrinology, oncology, and pharmacology. It serves as a key reference standard and intermediate for scientists and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors. Its precise chemical profile makes it essential for studies involving hormone-dependent processes and metabolic profiling.

Application

• Pharmaceutical Reference Standard: Used as a high-purity calibrant in analytical methods (HPLC, LC-MS) for drug development and quality control of hormone-based therapies.
• Biochemical Research: Critical for in-vitro and in-vivo studies investigating estrogen metabolism, receptor binding, and the mechanisms of hormone-related cancers.
• Diagnostic Kit Manufacturing: Serves as a key component or calibrator in the production of immunoassay kits for clinical testing of hormone levels.
• Metabolite Synthesis: Acts as a vital intermediate in the organic synthesis of more complex estrogen metabolites and related steroid compounds for research purposes.
• Toxicology & Safety Studies: Employed in metabolic pathway analysis and safety assessments of chemicals with potential endocrine-disrupting activity.

Basic Information

Product Name	4-Hydroxyestradiol
CAS No.	5976-61-4
Molecular Formula	C18H24O3
Molecular Weight	288.38 g/mol
Synonyms	Estra-1,3,5(10)-triene-3,4,17β-triol; 4,17β-Dihydroxyestra-1,3,5(10)-trien-3-ol; 4-OHE2; 4-OH-E2; 4-Hydroxy-17β-estradiol; 3,4,17β-Trihydroxyestra-1,3,5(10)-triene
EINECS	Contact for details

Quality Control

Our 4-Hydroxyestradiol is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including identity confirmation by spectroscopic methods and purity assessment by HPLC, to ensure it meets the stringent requirements for research and development applications. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Related Substances (HPLC)	Total impurities ≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.