Description

2''-Deoxy-5-Methylcytidine Hydrochloride is a modified nucleoside derivative, specifically the hydrochloride salt of 5-methyl-2'-deoxycytidine. This compound serves as a critical building block and research tool in the fields of molecular biology and medicinal chemistry. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and fine chemical manufacturers involved in oligonucleotide synthesis, epigenetics studies, and the development of novel therapeutics.

Application

• Oligonucleotide Synthesis: Used as a modified phosphoramidite precursor for incorporating 5-methylcytosine into synthetic DNA strands, crucial for epigenetic research and therapeutic oligonucleotides.
• Pharmaceutical Intermediates: Acts as a key starting material or intermediate in the synthesis of nucleoside-based antiviral and anticancer drug candidates.
• Biochemical Research: Employed in enzymatic studies, DNA methylation investigations, and as a substrate or inhibitor for methyltransferases and other nucleic acid-modifying enzymes.
• Diagnostic Probe Development: Utilized in the creation of labeled probes and primers for detecting methylation patterns in genomic DNA, relevant for diagnostic assays.
• Standards and References: Serves as a high-purity analytical standard for chromatography (HPLC, LC-MS) in quality control and metabolic studies.

Basic Information

Product Name	2''-Deoxy-5-Methylcytidine Hydrochloride
CAS No.	5241-10-1
Molecular Formula	C10H16N3O4 • HCl
Molecular Weight	277.71 g/mol (Free base: 241.25 g/mol)
Synonyms	5-Methyldeoxycytidine Hydrochloride; 5-Methyl-2'-deoxycytidine HCl; 5-Methyl-dC HCl; 2'-Deoxy-5-methylcytidine monohydrochloride; 5-Methyldeoxycytidine HCl; 5-Methylcytosine deoxyriboside hydrochloride; 5-Methyl-2'-deoxycytidine hydrochloride; d5mC HCl
EINECS	226-038-8

Quality Control

Our 2''-Deoxy-5-Methylcytidine Hydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture content. We provide Certificates of Analysis (COA) with each shipment, detailing all specifications and test results to ensure traceability and compliance with your research or cGMP-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain purity and prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Heavy Metals	≤10 ppm
Residual Solvents (GC)	Meets ICH guidelines
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.