Description

N(6)-Methyldeoxyadenosine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog, specifically methylated at the N6 position of the adenine base. This modification is critical for studying epigenetic regulation, DNA damage, and enzyme-substrate interactions, providing researchers with a powerful tool for probing nucleic acid structure and function. It is primarily utilized by research institutions, diagnostic developers, and biotechnology companies engaged in advanced molecular biology, enzymology, and next-generation sequencing applications.

Application

• Enzymology Research: Used as a substrate or inhibitor to study the activity and specificity of DNA polymerases, methyltransferases, and other nucleic acid-modifying enzymes.
• Epigenetics & Molecular Biology: Essential for in vitro studies on DNA methylation patterns, epigenetic signaling, and gene expression regulation.
• Next-Generation Sequencing (NGS): Serves as a modified nucleotide for developing novel sequencing-by-synthesis protocols and studying sequencing artifacts.
• Diagnostic Assay Development: Incorporated into probes or substrates for high-sensitivity detection kits targeting specific enzymatic activities or genetic markers.
• Structural Biology: Aids in X-ray crystallography and NMR studies to determine the three-dimensional structure of DNA-protein complexes.
• Chemical Biology & Drug Discovery: Used to create modified DNA strands for screening potential therapeutic compounds that target epigenetic readers or writers.

Basic Information

Product Name	N(6)-Methyldeoxyadenosine 5'-Triphosphate
CAS No.	5085-65-4
Molecular Formula	C₁₁H₁₈N₅O₁₂P₃
Molecular Weight	529.20 g/mol
Synonyms	N6-Methyldeoxyadenosine-5'-triphosphate; N6-Methyl-2'-deoxyadenosine-5'-triphosphate; 6-Methylaminopurine-2'-deoxyriboside-5'-triphosphate; 6-Methyl-dATP; N6-Methyl-dATP; 2'-Deoxy-N6-methyladenosine-5'-triphosphate; d(m6A)TP; N6-Methyl-2'-deoxyadenosine triphosphate
EINECS	Contact for details

Quality Control

Our N(6)-Methyldeoxyadenosine 5'-Triphosphate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to keep the material desiccated. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.