Description

7Beta-Hydroxycholesterol (CAS NO 566-27-8) is a significant oxysterol, a class of oxidized derivatives of cholesterol that play crucial roles in cellular signaling and metabolism. This compound serves as a valuable biochemical research standard and intermediate for studying lipid metabolism, oxidative stress, and related disease pathways. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and manufacturers of diagnostic kits and fine chemicals.

Application

• Biochemical Research Standard: Used as a reference standard in analytical methods (HPLC, GC-MS) for the quantification of oxysterols in biological samples.
• Pharmaceutical R&D: Employed in metabolic pathway studies, particularly research related to atherosclerosis, neurodegenerative diseases, and cancer.
• Diagnostic Reagent Intermediate: Serves as a key starting material or calibrant in the development of diagnostic assays for cholesterol oxidation products.
• Cell Biology Studies: Applied in vitro to investigate the effects of specific oxysterols on cell proliferation, apoptosis, and inflammatory responses.
• Metabolomics: A critical metabolite for mapping and understanding the cholesterol oxidation network in metabolomic profiling.
• Reference Material for Food & Cosmetic Analysis: Used to assess lipid oxidation levels and stability in complex matrices.

Basic Information

Product Name	7Beta-Hydroxycholesterol
CAS No.	566-27-8
Molecular Formula	C27H46O2
Molecular Weight	402.66 g/mol
Synonyms	(3β)-Cholest-5-ene-3,7-diol; 7β-Hydroxycholesterol; 7β-HC; (3β,7β)-Cholest-5-ene-3,7-diol; 5-Cholestene-3β,7β-diol; 7β-Hydroxy-cholest-5-en-3β-ol; NSC 338989
EINECS	209-293-6

Quality Control

Our 7Beta-Hydroxycholesterol is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure identity, purity, and consistency. We provide a detailed Certificate of Analysis (COA) with key parameters including assay by HPLC, identification by FT-IR, and specified impurity limits. Our quality commitment supports applications requiring reliable and traceable reference materials for research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. For long-term storage, keep desiccated at -20°C. The container should be kept in a cool, dry, and well-ventilated area away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Solubility	Soluble in ethanol, DMSO; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.